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http://dx.doi.org/10.3807/COPP.2022.6.1.086

Autofluorescence Loss in Photobleaching for Human Dentin ex vivo  

Lee, Seunghwan Goldmund (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University)
Kim, Minwoo (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University)
Jeong, Sunghee (Department of Oral Medicine, School of Dentistry, Pusan National University)
Hwang, Jaejoon (Department of Oral and Maxillofacial Radiology, School of Dentistry, Pusan National University, Dental and Life Science Institute)
Kim, Jisu (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University)
Gourrier, Aurelien (University Grenoble Alpes, Laboratoire Interdisciplinaire de Physique)
Vial, Jean Claude (University Grenoble Alpes, Laboratoire Interdisciplinaire de Physique)
Kyhm, Kwangseuk (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University)
Publication Information
Current Optics and Photonics / v.6, no.1, 2022 , pp. 86-91 More about this Journal
Abstract
Two-photon fluorescence microscopy was performed on the enamel-dentin junction area of a human tooth using a femtosecond pulsed laser. We obtained a clear image contrast between the bright dentin and dark tubules with the autofluorescence generated from the endogenous fluorophores in dentin. The autofluorescence shows a broad spectrum due to complex cross links between dentinal collagens, which extend from blue to orange wavelengths (470-590 nm), but a gradual autofluorescence loss in photobleaching was observed for a long-term exposure under strong excitation. For increasing excitation power, we found that two-step decay becomes significant in the spectrally integrated autofluorescence.
Keywords
Autofluorescence; Dentin; Fluorescence loss in photobleaching; Two-photon fluorescence microscopy;
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