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http://dx.doi.org/10.3807/COPP.2018.2.6.595

High-speed Two-photon Laser Scanning Microscopy Imaging of in vivo Blood Cells in Rapid Circulation at Velocities of Up to 1.2 Millimeters per Second  

Boutilier, Richard M. (School of Mechanical Engineering, Kyungpook National University)
Park, Jae Sung (School of Mechanical Engineering, Kyungpook National University)
Lee, Ho (School of Mechanical Engineering, Kyungpook National University)
Publication Information
Current Optics and Photonics / v.2, no.6, 2018 , pp. 595-605 More about this Journal
Abstract
The two-photon process of microscopy provides good spatial resolution and optical sectioning ability when observing quasi-static endogenous fluorescent tissue within an in vivo animal model skin. In order to extend the use of such systems, we developed a two-photon laser scanning microscopy system capable of also capturing $512{\times}512$ pixel images at 90 frames per second. This was made possible by incorporating a 72 facet polygon mirror which was mounted on a 55 kRPM motor to enhance the fast-scan axis speed in the horizontal direction. Using the enhanced temporal resolution of our high-speed two-photon laser scanning microscope, we show that rapid processes, such as fluorescently labeled erythrocytes moving in mouse blood flow at up to 1.2 mm/s, can be achieved.
Keywords
Scanning microscopy; Nonlinear microscopy; Fluorescence microscopy; Biological imaging;
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