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http://dx.doi.org/10.3807/KJOP.2015.26.1.023

Study of a Brain Tumor and Blood Vessel Detection System Using Multiple Fluorescence Imaging by a Surgical Microscope  

Lee, Hyun Min (Biomedical Engineering Branch, Division of Convergence Technology, National Cancer Center)
Kim, Hong Rae (Biomedical Engineering Branch, Division of Convergence Technology, National Cancer Center)
Yoon, Woong Bae (Biomedical Engineering Branch, Division of Convergence Technology, National Cancer Center)
Kim, Young Jae (Biomedical Engineering Branch, Division of Convergence Technology, National Cancer Center)
Kim, Kwang Gi (Biomedical Engineering Branch, Division of Convergence Technology, National Cancer Center)
Kim, Seok Ki (Molecular Imaging & Therapy Branch, National Cancer Center)
Yoo, Heon (Specific Organs Cancer Branch & Hospital, National Cancer Center)
Lee, Seung Hoon (Specific Organs Cancer Branch & Hospital, National Cancer Center)
Shin, Min Sun (Reserch & Development Planning Department, Bionet)
Kwon, Ki Chul (Department of Electrical and Computer Engineering, Chungbuk National University)
Publication Information
Korean Journal of Optics and Photonics / v.26, no.1, 2015 , pp. 23-29 More about this Journal
Abstract
In this paper, we propose a microscope system for detecting both a tumor and blood vessels in brain tumor surgery as fluorescence images by using multiple light sources and a beam-splitter module. The proposed method displays fluorescent images of the tumor and blood vessels on the same display device and also provides accurate information about them to the operator. To acquire a fluorescence image, we utilized 5-ALA (5-aminolevulinic acid) for the tumor and ICG (Indocyanine green) for blood vessels, and we used a beam-splitter module combined with a microscope for simultaneous detection of both. The beam-splitter module showed the best performance at 600 nm for 5-ALA and above 800 nm for ICG. The beam-splitter is flexible to enable diverse objective setups and designed to mount a filter easily, so beam-splitter and filter can be changed as needed, and other fluorescent dyes besides 5-ALA and ICG are available. The fluorescent images of the tumor and the blood vessels can be displayed on the same monitor through the beam-splitter module with a CCD camera. For ICG, a CCD that can detect the near-infrared region is needed. This system provides the acquired fluorescent image to an operator in real time, matching it to the original image through a similarity transform.
Keywords
Fluorescence microscope; Brain surgery; 5-ALA; ICG; Beam splitter;
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