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http://dx.doi.org/10.3807/HKH.2009.20.1.053

Study on Refractive Index and Thickness of Human Stem Cells by Using Imaging Ellipsometry  

Choi, Joong-Kyu (Ellipsometry Lab. & Brain Disease Research Center, Department of Molecular Science & Technology, Ajou University)
Shim, Woo-Young (Ellipsometry Lab. & Brain Disease Research Center, Department of Molecular Science & Technology, Ajou University)
Lee, Gwang (Ellipsometry Lab. & Brain Disease Research Center, Department of Molecular Science & Technology, Ajou University)
Kim, Sang-Youl (Ellipsometry Lab. & Brain Disease Research Center, Department of Molecular Science & Technology, Ajou University)
Park, Sang-Uk (Department of Research and Development, Ellipso Technology Co., Ltd.)
CheGal, Won (Nanobio Fusion Research Center, Korea Research Institute of Standards and Science)
Cho, Hyun-Mo (Nanobio Fusion Research Center, Korea Research Institute of Standards and Science)
Cho, Yong-Jai (Nanobio Fusion Research Center, Korea Research Institute of Standards and Science)
Publication Information
Korean Journal of Optics and Photonics / v.20, no.1, 2009 , pp. 53-56 More about this Journal
Abstract
We applied an ellipsometric technique to get quantitative information about the thickness and refractive index of human Mesenchymal Stem Cells (hMSCs). The images of ellipsometric constants $\Delta$, $\Psi$ for the nucleus region and for the cell body region of hMSCs were obtained by using an Imaging Ellipsomter (IE) for their in vitro state. A numerical inversion method was applied to deduce the refractive index and the thickness of hMSCs from the measured $\Delta$, $\Psi$. Thus the images of the refractive index and those of the thickness of hMSCs for the nucleus region and for the cell body region are reported.
Keywords
Ellipsometry; Imaging Ellipsometer; Stem Cells; MSCs; Cell Thickness; Refractive Index; Image;
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