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http://dx.doi.org/10.7852/jses.2014.52.2.102

Production of the yellow fluorescent silk using the fibroin heavy chain protein expression system in transgenic silkworm  

Kim, Seong Wan (National Academy of Agricultural Science, RDA)
Choi, Kwang-Ho (National Academy of Agricultural Science, RDA)
Kim, Seong Ryul (National Academy of Agricultural Science, RDA)
Yun, Eun Young (National Academy of Agricultural Science, RDA)
Park, Seung Won (Department of Biotechnology, Catholic University of Daegu)
Kang, Seok Woo (National Academy of Agricultural Science, RDA)
Goo, Tae Won (Department of Biochemistry, School of Medicine, Dongguk University of Gyeongju)
Publication Information
Journal of Sericultural and Entomological Science / v.52, no.2, 2014 , pp. 102-109 More about this Journal
Abstract
We constructed the fibroin H-chain expression system to produce enhanced yellow fluorescent proteins (EYFP) in the silk of transgenic silkworm. Fluorescent silk could be made by fusing EYFP cDNA to the heavy chain gene and injecting it into a silkworm. The EYFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EYFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The yellow fluorescence proving that the fusion protein was present in the silk. Accordingly, we suggest that the EYFP fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.
Keywords
Bombyx mori; Silk; Fibroin; EYFP; Fluorescence; Transgenic silkworm;
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