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http://dx.doi.org/10.7852/jses.2013.51.2.185

Taxonomy of introduced commercial insect, Zophobas atratus (Coleoptera: Tenebrionidae) and a comparison of DNA barcoding with similar tenebrionids, Promethis valgipes and Tenebrio molitor in Korea  

Park, Hae Chul (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Jung, Boo Hee (Korean Entomological Institute, Korea University)
Han, Taeman (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Lee, Young Bo (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Kim, Seong-Hyun (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Kim, Nam Jeong (Applied Entomology Division, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Sericultural and Entomological Science / v.51, no.2, 2013 , pp. 185-190 More about this Journal
Abstract
The superworm, as known the larva of Zophobas morio, has been officially imported from 2011 and bred commercially in Korea. But it is named as the corrected scientific name, Zophobas atratus by junior synonym throughout traditional taxonomy in this study and newly designated Korean name as 'a-me-ri-ca-wang-geo-jeo-ri' in terms of resource management. Z. atratus was compared with wild native tenebrionids, Promethis valgipes and a commercial reared Tenebrio molitor on the basis of DNA barcode analysis. As the results, the average genetic divergence was 21.4% between Z. atratus and P. valgipes, and 20.9% between Z. atratus and T. molitor. These large divergences imply these tenebrionids species can be easily identified by DNA barcodes. The results of genetic divergences within species also suggest that Korean populations of Z. atratus, having the same haplotype, might be introduced from the same area of foreign country. On the other hand, a population of T. molitor was separated into two distinct intra-specific groups with DNA barcoding gaps ranged from 1.17- 2.19%. We suppose that domestic breeding entities of T. molitor might be introduced and mixed from two different local groups. Through this study, we expect that classification for two tenebrionid introduced from foreign countries can be used for the management of insect resources in Korea.
Keywords
Coleoptera; Tenebrionidae; Zophobas atratus; Tenebrio molitor; Promethis valgipes; Morphology; DNA barcode;
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1 Bang SW, Kim MH, Rho TH (2004) Development of integrated management plan for abating the threats from invasive alien species in Korea. pp 313. Korean Environmental Institute, Seoul.
2 Bednarova M, Borkovcova M, Komprda T (2013) Purine derivate content and amino acid profile in larval stages of three edible insects. J Sci Food Agric DOI 10.1002/jsfa.6198.   DOI
3 Chen WT, Liu HL, Yao LG, Ye ZL, Guo YW (2012) Assay and comparison of chemical constituents of crude methanol extracts from different developmental stages of darkling beetle Zophobas morio (Coleoptera: Tenebrionidae). Acta Entomologica Sinica 5593, 357-360.
4 Animal and Plant Qurantine Agency. [Online] Insect pests and pet insects. http://www.qia.go.kr/plant/pest/plant_insec_rule.jsp#this.
5 Choi YC, Song HS (2011) Mass rearing and application technique of mealworm, Tenebrio molitor, pp 14-18. Applied entomology Division, NAAS Press, Korea.
6 Ebeling W (1975) Urban entomology. Pest of stored food products. pp 275-309. Division of Agriculture Sciences, University of California. Berkeley, USA.
7 Friederich U, Volland W (1992) Futtertierzucht (2 ed). pp 188. Ulmer, Stuttgart.
8 Gebien, H (1941) Katalog der Tenebrioniden. Teil II. [Part.] Mitt Munch Entomol Ges 31: 331-362 (626-657), 803-834 (658-689), 1131-1146 (690-705).
9 Hebert PDN, Cywinska A, Ball SL, deWaard JR (2003) Biological identifications through DNA barcodes. Proc R Soc Lond B 270, 313-321.   DOI   ScienceOn
10 Ichikawa T, Sakamoto H (2013) A third type of defensive behavior in the tenebrionid beetle Zophobas atratus pupae. J Insect Sci 13(33), 1-12.
11 Jahnke M (2005) Gregarina tibengae sp. n. (Apicomplexa: Eugregarinida) described from Zophobas atratus Fabricius, 1775 (Coleoptera: Tenebrionidae). Acta Protozool 44, 67-74.
12 Jung BH (2012) Insect fauna of Korea 12, 5. Darkling beetles (Coleoptera: Tenebrionidae:Tenebrioninae). pp 123. Flora and Fauna of Korea, National Institute of Biological Resources Press, Korea.
13 Ministry of Agriculture, Food and Rural Affairs. [Online] Information for laws. http://www.mafra.go.kr/list.jsp?group_id=2&menu_id=33&link_menu_id=47&division=H&board_kind=&board_skin_id=&parent_code=2&link_url=&depth=1&tab_yn=N&code=top.
14 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28, 2731-2739.   DOI   ScienceOn
15 Tschinkel R (1984) Zophobas atratus (Fab.) and Z. rugipes Kirsch (Coleoptera: Tenebrionidae) are the same species. Coleop Bull 38(4), 325-333.
16 Jabir MDAR, Razak SA, Vikineswary S (2012) Nutritive potential and utilization of super worm (Zophobas morio) meal in the diet of nile tilapia (Oreochromis niloticus) juvenile. Afr J Biotechnol 11, 6592-6598.
17 Yen SH (2009) Comprehensive checklist and database reinforcement of exotic animals with high risk in Taiwan. Project Number: 101 Lin Fa-07.1-Paul44. pp.233. In: Council of Agriculture, Executive Yuan and its subordinate agencies overseeing technology programs 100 year detailing / Final report of single project (in Chinese).
18 Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3, 294-299.