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http://dx.doi.org/10.7852/jses.2012.50.2.161

Antimicrobial activity and characterization for defensin of synthetic oligopeptides derived from Bombus ignitus  

Kang, Heui-Yun (Gyeonggido Agricultural research and Extension Services)
Kim, In-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Lee, Joon-Ha (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kwon, Young Nam (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Yoon, Hyung Joo (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kim, Iksoo (Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University)
Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Journal of Sericultural and Entomological Science / v.50, no.2, 2012 , pp. 161-165 More about this Journal
Abstract
Antimicrobial peptides of insects are found and reported as immune defence system against infectious agents. The peptides are produced by fat body cells and thrombocytoids, a blood cell type. Defensin is 38-45 amino acids long and consists of an ${\alpha}$-helix linked by a loop to an antiparallel ${\beta}$-sheet. Defensin from a bumblebee, Bombus ignitus, is known to comprise 52 amino acid residues. This peptide consists of two ${\alpha}$-helixes; ACAANCLSM and KTNFKDLWDKRF and one ${\beta}$-sheet; GGRCENGVCLCR. We carried out antibacterial activity test by radial diffusion assay against Staphylococcus aureus (Gram positive), Escherichia coli (Gram negative), Pseudomonas syringae (Gram negative), Candida albicans (fungi), MDRPA, MRSA, and VRE (antimicrobial resistant microbes) with synthetic oligopeptides from Peptron (Daejeon, Korea). The predicted curtailment fragment (GGRCEVCLCR-$NH_2$) for ${\beta}$-sheet had strong antibacterial activity when internal amino acids were removed. But, curtailment fragments (ACAANCLSM-$NH_2$ and TNFKDLWDKR-$NH_2$) of ${\alpha}$-helix were not showed antibacterial activity. These synthetic oligopeptides were showed the great activity against Gram positive and negative bacteria.
Keywords
Antimicrobial peptides; Defensin; Bombus ignitus;
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