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Effect of Silkworm Hemolymph Collected Large-scale Bleeding Method in Insect Cell Culture  

Choi, Ji-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, R.D.A.)
Kim, Jong-Gill (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, R.D.A.)
Choi, Young-Cheol (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, R.D.A.)
Kim, Sam-Eun (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, R.D.A.)
Han, Myung-Sae (Kyungpook National University)
Publication Information
Journal of Sericultural and Entomological Science / v.49, no.1, 2007 , pp. 28-32 More about this Journal
Abstract
This study was carried out to investigate the utilization of hemolymph of silkworm, Bombyx mori, as a substitute for fetal bovine serum(FBS) in the insect cell culture. Hemolymph is collected on a small scale by clipping the abdominal leg; however, this method is not appropriate for large scale collection. The hemolymph was collected from 5 th instar larva by centrifugation after cutting of the abdominal legs was more appropriate procedure for large scale collection. The cell growth in the medium supplemented with hemolymph(Baekokjam) collected in large scale was almost same as that in the medium hemolymph supplemented with hemolymph collected in small scale. However, the mutant($wE^b$) hemolymph collected in large scale was still less effective in the cell growth, as compared to the Baekokjam hemolymph collected in large scale. The optimum centrifugation condition for large-scale bleeding was 500 rpm and 15 min.
Keywords
Bombyx mori; hemolymph; insect cell; FBS; Silkworm;
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