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http://dx.doi.org/10.7732/kjpr.2022.35.2.372

Verification of the Effect of Lemon Balm Extract on Triglyceride Control According to the Extraction Solvent  

Kim, Ji Youn (SEOUL F&B CO., LTD.)
Kim, Kyoung Kon (Well-being Bioproducts R&D Center, Kangwon National University)
Lee, Hye Rim (Well-being Bioproducts R&D Center, Kangwon National University)
Kim, Dae Jung (Well-being Bioproducts R&D Center, Kangwon National University)
Kim, Tae Woo (Well-being Bioproducts R&D Center, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.35, no.2, 2022 , pp. 372-379 More about this Journal
Abstract
This study investigated the effect of lemon balm (Melissa officinalis) extract on improving blood triglycerides according to the extraction solvent using 3T3-L1 cells. Lemon balm was extracted with water (MOW100), 70% ethanol (MOE70), 50% ethanol (MOE50), and 30% ethanol (MOE30). To verify its efficacy on improving blood triglycerides, cell viability, lipid accumulation, triglyceride (TG) content, and expressions of protein kinase A (PKA), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), perilipin, and CGI-58 proteins were evaluated. Cytotoxicity was not evident up to an extract concentration of 1 mg/mL. Lipid accumulation and TG content were decreased in a concentration-dependent manner compared to their levels in the control group. When the MOW100 extract was applied at a concentration of 0.2 mg/mL, an inhibitory effect was evident, with lipid accumulation inhibited by 21.3% and TG content reduced by 32.7%. PKA phosphorylation and ATGL HSL and CGI-58 levels were increased. The data indicate that lemon balm extract obtained using water is more efficacious than extracted with ethanol. The aqueous extract shows potential in triglyceride control through lipolysis and lowering triglyceride levels.
Keywords
ATGL; HSL; Melissa officinalis; PKA; Triglyceride;
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