Characterization of Phenotypic Traits and Evaluation of Glucosinolate Contents in Radish Germplasms (Raphanus sativus L.) |
Kim, Bichsaem
(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
Hur, Onsook (National Agrobiodiversity Center, National Institute of Agricultural Sciences) Lee, Jae-Eun (National Agrobiodiversity Center, National Institute of Agricultural Sciences) Assefa, Awraris Derbie (National Agrobiodiversity Center, National Institute of Agricultural Sciences) Ko, Ho-Cheol (Client Service Division, Planning and Coordination Bureau, RDA) Chung, Yun-Jo (National Creative Research Laboratory for Ca2+ Signaling Network, Jeonbuk National University Medical School) Rhee, Ju-hee (National Agrobiodiversity Center, National Institute of Agricultural Sciences) Hahn, Bum-Soo (National Agrobiodiversity Center, National Institute of Agricultural Sciences) |
1 | Jatoi, S.A., S.U. Siddiqui, M.S. Masood, A. Javaid, M. Iqbal and O.U. Sayal. 2011. Genetic diversity in radish germplasm for morphological traits and seed storage proteins. Pakistan J. Bot. 43:2259-2268. |
2 | Kwon, S.T., I.Y. and J.H. Shin. 2020. Investigation of defense and vegetative growth related traits of recombinant inbred lines of Brassica rapa. Korean J. Plant Res. 33:615-623. DOI |
3 | Liu, M., L. Zhang, S.L. Ser, J.R. Cumming and K.M. Ku. 2018. Comparative phytonutrient analysis of broccoli by-products: The potentials for broccoli by-product utilization. Molecules 23:900. DOI |
4 | Montaut, S., J. Barillari, R. Iori and P. Rollin. 2010. Glucoaphasatin: Chemistry, occurrence, and biological properties. Phytochemistry 71:6-12. DOI |
5 | Rajalingam, N., J.H. Yoon, B. Yoon, N.B. Hung, W.I. Kim, H. Kim, B.Y. Park and S.R. Kim. 2021. Prevalence and molecular characterization of Escherichia coli isolates during radish sprout production in the Republic of Korea. Appl. Biol. Chem. 64:1-8. |
6 | Rhee, J.H., S. Choi, J.E. Lee, O.S. Hur, N.Y. Ro, A.J. Hwang, H.C. Ko, Y.J. Chung, J.J. Noh and A.D. Assefa. 2020. Glucosinolate content in Brassica genetic resources and their distribution pattern within and between inner, middle, and outer leaves. Plants 9:1421. DOI |
7 | Suzuki, I., Y.M. Cho, T. Hirata, T. Toyoda, J.I. Akagi, Y. Nakamura, A. Sasaki, T. Nakamura, S. Okamoto and K. Shirota. 2017. Toxic effects of 4-methylthio-3-butenyl isothiocyanate (Raphasatin) in the rat urinary bladder without genotoxicity. J. Appl. Toxicol. 37:485-494. DOI |
8 | Barillari, J., D. Canistro, M. Paolini, F. Ferroni, G.F. Pedulli, R. Iori and L. Valgimigli. 2005. Direct antioxidant activity of purified glucoerucin, the dietary secondary metabolite contained in rocket (Eruca sativa Mill.) seeds and sprouts. J. Agr. Food Chem. 53:2475-2482. DOI |
9 | Brand-Williams, W., M.E. Cuvelier and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Tech. 28:25-30. DOI |
10 | Cai, Y.X., J.H. Wang, C. McAuley, M.A. Augustin and N.S. Terefe. 2019. Fermentation for enhancing the bioconversion of glucoraphanin into sulforaphane and improve the functional attributes of broccoli puree. J. Functional Foods 61:103461. DOI |
11 | Cho, W.K. 2010. A historical study of Korean traditional radish kimchi. J. Korean Soc. Food Cult. 25:428-455. |
12 | De Nicola, G.R., M. Bagatta, E. Pagnotta, D. Angelino, L. Gennari, P. Ninfali, P. Rollin and R. Iori. 2013. Comparison of bioactive phytochemical content and release of isothiocyanates in selected Brassica sprouts. Food Chem. 141:297-303. DOI |
13 | Scholl, C., B.D. Eshelman, D.M. Barnes and P.R. Hanlon. 2011. Raphasatin is a more potent inducer of the detoxification enzymes than its degradation products. J. Food Sci. 76:C504-C511. DOI |
14 | International Board for Plant Genetic Resources (IBPGR). 1990. Descriptors for Brassica and Raphanus, https://www.bioversityinternational.org/e-library/publications/detail/descriptors-for-brassica-and-raphanus/ (Accessed on 10 Oct, 2021) |
15 | Ishida, M., T. Kakizaki, Y. Morimitsu, T. Ohara, K. Hatakeyama, H. Yoshiaki, J. Kohori and T. Nishio. 2015. Novel glucosinolate composition lacking 4-methylthio-3-butenyl glucosinolate in Japanese white radish (Raphanus sativus L.). Theor. Appl. Genet. 128: 2037-2046. DOI |
16 | Gratacos-Cubarsi, M., A. Ribas-Agusti, J.A. Garcia-Regueiro and M. Castellari. 2010. Simultaneous evaluation of intact glucosinolates and phenolic compounds by UPLC-DAD-MS/MS in Brassica oleracea L. var. botrytis. Food Chem. 121:257-263. DOI |
17 | Hwang, I.M., B. Park, Y.M. Dang, S.Y. Kim and H.Y. Seo. 2019. Simultaneous direct determination of 15 glucosinolates in eight Brassica species by UHPLC-Q-Orbitrap-MS. Food Chem. 282:127-133. DOI |
18 | Yi, G., S. Lim, W.B. Chae, J.E. Park, H.R. Park, E.J. Lee and J.H. Huh. 2016. Root glucosinolate profiles for screening of radish (Raphanus sativus L.) genetic resources. J. Agr. Food Chem. 64:61-70. DOI |
19 | Vavilov, N.I. 1926. Studies on the origin of cultivated plants. Bull. Appl. Bot. Plant Breeding 14:1-245. |
20 | Liang, H., Y. Wei, R. Li, L. Cheng, Q. Yuan and F. Zheng. 2018. Intensifying sulforaphane formation in broccoli sprouts by using other cruciferous sprouts additions. Food Sci. Biotech. 27:957-962. DOI |
21 | Park, S., M.V. Arasu, M.K. Lee, J.H. Chun, J.M. Seo, S.W. Lee, N.A. Al-Dhabi and S.J. Kim. 2014. Quantification of glucosinolates, anthocyanins, free amino acids, and vitamin C in inbred lines of cabbage (Brassica oleracea L.). Food Chem. 145:77-85. DOI |
22 | Singh, B., T. Koley, P. Karmakar, A. Tripathi, B. Singh and M. Singh. 2017. Pigmented radish (Raphanus sativus): Genetic variability, heritability and interrelationships of total phenolics, anthocyanins and antioxidant activity. Indian J. Agr. Sci. 87:1600-1606. |
23 | Suzuki, I., Y.M. Cho, T. Hirata, T. Toyoda, J.I. Akagi, Y. Nakamura, E.Y. Park, A. Sasaki, T. Nakamura and S. Okamoto. 2016. 4-Methylthio-3-butenyl isothiocyanate (raphasatin) exerts chemopreventive effects against esophageal carcinogenesis in rats. J. Toxicol. Pathol. 29:237-246. DOI |
24 | International Union for the Protection of new Varieties of plants (UPOV). 2021. For distictess, uniformity and stability, https://www.upov.int/edocs/mdocs/upov/en/twv/44/tg_63_7_proj_4_and_64_7_proj_3.pdf (Accessed on 10 Oct, 2021). |
25 | Wang, Q., Y. Wang, H. Sun, L. Sun and L. Zhang. 2020. Transposon-induced methylation of the RsMYB1 promoter disturbs anthocyanin accumulation in red-fleshed radish. J. Exp. Bot. 71: 2537-2550. DOI |
26 | Bhandari, S.R., J.S. Jo and J.G. Lee. 2015. Comparison of glucosinolate profiles in different tissues of nine Brassica crops. Molecules 20:15827-15841. DOI |
27 | Afroz, T., O. Hur, N. Ro, J.E. Lee, A. Hwang, B. Kim, A.D. Assefa, J.H. Rhee, J.S. Sung and H.S. Lee. 2021. Evaluation of bioassay methods to assess bacterial soft rot resistance in radish cultivars. J. Life Sci. 31:609-616. DOI |
28 | Jeon, B.W., M.H. Oh, E.O. Kim, H.S. Kim and W.B. Chae. 2018. Different vegetative growth stages of kimchi cabbage (Brassica rapa L.) exhibit specific glucosinolate composition and content. Hortic. Envir. Biotech. 59:355-362. DOI |
29 | Aires, A., V. Mota, M. Saavedra, E. Rosa and R. Bennett. 2009. The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract. J. Appl. Microbiol. 106:2086-2095. DOI |
30 | Fahey, J.W., A.T. Zalcmann and P. Talalay. 2001. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5-51. DOI |
31 | Geum, N.G., J.H. Yeo, J.H. Yu, M.Y. Choi, J.W. Lee, J.K. Baek and J.B. Jeong. 2021. In vitro immunostimulatory activity of Bok Choy (Brassica campestris var. chinensis) sprouts in RAW264.7 macrophage cells. Korean J. Plant Res. 34:203-215. DOI |
32 | Heo, Y., M.J. Kim, J.W. Lee and B. Moon. 2019. Muffins enriched with dietary fiber from kimchi by-product: Baking properties, physical-chemical properties, and consumer acceptance. Food Sci. Nutr. 7:1778-1785. DOI |
33 | Ibrahim, M.D., S.B. Kntayya, N. Mohd Ain, R. Iori, C. Ioannides and A.F. Abdull Razis. 2018. Induction of apoptosis and cytotoxicity by raphasatin in human breast adenocarcinoma MCF-7 cells. Molecules 23:3092. DOI |
34 | Kakizaki, T., H. Kitashiba, Z. Zou, F. Li, N. Fukino, T. Ohara, T. Nishio and M. Ishida. 2017. A 2-oxoglutarate-dependent dioxygenase mediates the biosynthesis of glucoraphasatin in radish. Plant Physiol. 173:1583-1593. DOI |
35 | Kurina, A.B., D.L. Kornyukhin, A.E. Solovyeva and A.M. Artemyeva. 2021. Genetic diversity of phenotypic and biochemical traits in VIR radish (Raphanus sativus L.) germplasm collection. Plants 10:1799. DOI |
36 | Kim, M.J., H.J. Yang, H.Y. Lee, Y.M. Park, D.Y. Shin, Y.H. Lee, Y.G. Kang, T.S. Kim, S.P. Lee and K.H. Park. 2020. Improving effects of Brassica oleraceae L. var. italica sprout extract on alcohol liver dysfunction. Korean J. Plant Res. 33:163-169. DOI |
37 | Kaneko,Y. and Y. Matsuzawa. 1993. Radish (Raphanus sativus L.), p. 487-505. In: G. Kalloo and B.O. Bergh (eds). Genetic improvement of vegetable crops. Pergamon Press, Oxford, England. |
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