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http://dx.doi.org/10.7732/kjpr.2021.34.4.377

Effect of Fruits from Pyrus ussuriensis var. hakunensis (Nakai) T.B. Lee on Macrophage Activation  

Geum, Na Gyeong (Department of Medicinal Plant Resources, Andong National University)
Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University)
Publication Information
Korean Journal of Plant Resources / v.34, no.4, 2021 , pp. 377-383 More about this Journal
Abstract
In this study, we investigated in vitro immunostimulatory activity of fruit extracts from Pyrus ussuriensis var. hakunensis (Nakai) T.B. Lee (PUF) using mouse macrophage RAW264.7 cells. PUF increased the production of immunostimulatory factors such as NO, iNOS, IL-1β, IL-6 and TNF-α, and phagocytic activity in RAW264.7 cells. The inhibition of TLR2 and TLR4 blocked PUF-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of MAPKs signaling pathway reduced PUF-mediated production of immunostimulatory factors. From these results, PUF may have immunostimulatory activity via TLR2/4-mediated activation of MAPKs signaling pathway. Therefore, PUF expected to be used as a potential immune-enhancing agent.
Keywords
Immunity; Immunostimulatory activity; Macrophage activation; Pyrus ussuriensis;
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1 Toussi, D.N. and P. Massari. 2014. Immune adjuvant effect of molecularly-defined toll-like receptor ligands. Vaccines (Basel) 2(2):323-353.   DOI
2 Alagawany, M., Y.A. Attia, M.R. Farag, S.S. Elnesr, S.A. Nagadi, M.E. Shafi, A.F. Khafaga, H. Ohran, A.A. Alaqil and M.E. Abd El-Hack. 2021. The strategy of boosting the immune system under the COVID-19 pandemic. Front. Vet. Sci. 7:570748.   DOI
3 Wada, T. and J.M. Penninger. 2004. Mitogen-activated protein kinases in apoptosis regulation. Oncogene 23:2838-2849.   DOI
4 Wang, Y., Y. Tian, J. Shao, X. Shu, J. Jia, X. Ren and Y. Guan. 2018. Macrophage immunomodulatory activity of the polysaccharide isolated from Collybia radicata mushroom. Int. J. Biol. Macromol. 108:300-306.   DOI
5 Zhang, C., N. Bai, A. Chang, Z. Zhang, J. Yin, W. Shen, Y. Tian, R. Xiang and C. Liu. 2013. ATF4 is directly recruited by TLR4 signaling and positively regulates TLR4-trigged cytokine production in human monocytes. Cell. Mol. Immunol. 10:84-94.   DOI
6 Kawasaki, T. and T. Kawai. 2014. Toll-like receptor signaling pathways. Front. Immumol. 5:461.
7 Barbara, J.A., X. Van Ostade and A.F. Lopez. 1996. Tumour necrosis factor-alpha (TNF-α): the good, the bad and potentially very effective. Immunol. Cell Biol. 74:434-443.   DOI
8 Foley, E. and P.H. O'Farrell. 2003. Nitric oxide contributes to induction of innate immune responses to gram-negative bacteria in Drosophila. Gene Dev. 17:115-125.   DOI
9 Janeway, C.A. Jr. and R. Medzhitov. 2002. Innate immune recognition. Annu. Rev. Immunol. 20:197-216.   DOI
10 Kim, B.H., W.J. Lee, B. Sanjel, K. Cho, Y.K. Son, H.Y. Park, S.Y. Kim and W.S. Shim. 2019. Extracts of the leaves of Pyrus ussuriensis Maxim. Alleviate itch sensation via TSLP-dependent manner in mouse models of atopic dermatitis. Physiol. Behav. 210:112624.   DOI
11 Mills, C.D. and K. Ley. 2014. M1 and M2 macrophages: the chicken and the egg of immunity. J. Innate Immun. 6:716-726.   DOI
12 Lee, J.C., J.T. Laydon, P.C. McDonnell, T.F. Gallagher, S. Kumar, D. Green, D. McNulty, M.J. Blumenthal, J.R. Heys, S.W. Landvatter, J.E. Strickler, M.M. McLaughlin, I.R. Siemens, S.M. Fisher, G.P. Livi, J.R. White, J.L. Adams and P.R. Young. 1994. A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature 372:739-746.   DOI
13 Lee, K.H., J.Y. Cho, H.J. Lee, Y.K. Ma, J. Kwon, S.H. Park, S.H. Lee, J.A. Cho, W.S. Kim, K.H. Park and J.H. Moon. 2011. Hydroxycinnamoylmalic acids and their methyl esters from pear (Pyrus pyrifolia Nakai) fruit peel. J. Agric. Food Chem. 59(18):10124-10128.   DOI
14 Li, X., T. Wang, B. Zhou, W. Gao, J. Cao and L. Huang. 2014. Chemical composition and antioxidant and anti-inflammatory potential of peels and flesh from 10 different pear varieties (Pyrus spp). Food Chem. 152:531-538.   DOI
15 Mills, C.D., L.L. Lenz and K. Ley. 2015. Macrophages at the fork in the road to health or disease. Front. Immunol. 6:59.   DOI
16 Seo, H.J. and J.B. Jeong. 2020. Immune-enhancing effects of green lettuce (Lactuca sativa L.) extracts through the TLR4-MAPK/NF-κB signaling pathways in RAW264.7 macrophage cells. Korean J. Plant Res. 33(3):183-193.   DOI
17 Kim, H., T.H. Kim, B. Jeon, M.H. Bang, W.J. Kim, J.W. Park and D.K. Chung. 2020. Evaluation of the physiological activity on the skin and identification of the active ingredient of leaf extract from Sanhyang sandolbae (Pyrus ussuriensis) as a new variety. J. Korean Soc. Food Sci. Nutr. 49(1):35-45.   DOI
18 Reiling, N., A. Blumenthal, H.D. Flad, M. Ernst and S. Ehlers. 2001. Mycobacteria-induced TNF-alpha and IL-10 formation by human macrophages is differentially regulated at the level of mitogen-activated protein kinase activity. J. Immunol. 167:3339-3345.   DOI
19 Scheller, J., A. Chalaris, D. Schmidt-Arras and S. Rose-John. 2011. The pro- and anti-inflammatory properties of the cytokine interleukin-6. Biochim. Biophys. Acta. 1813:878-888.   DOI
20 Seillet, C., G.T. Belz and L.A. Mielke. 2014. Complexity of cytokine network regulation of innate lymphoid cells in protective immunity. Cytokine 70:1-10.   DOI
21 Tosi, M.F. 2005. Innate immune responses to infection. J. Allergy Clin. Immunol. 116(2):241-249.   DOI
22 Qu, J.-H., B. Du, F. Peng, T.-K. Wang and Y.-D. Yang. 2016. Optimisation of triterpenoids extraction from Anli pears (Pyrus ussuriensis Maxim) by pressurised liquid extraction. Qual. Assur. Saf. Crops Foods 8:105-110.   DOI
23 Duque, G.A. and A. Descoteaux. 2014. Macrophage cytokines: involvement in immunity and infectious diseases. Front. Immunol. 5:491.   DOI
24 Neurath, M.F. 2014. Cytokines in inflammatory bowel disease. Nat. Rev. Immunol. 15:329-342.   DOI
25 Nick, J.A., N.J. Avdi, P. Gerwins, G.L. Johnson and G.S. Worthen. 1996. Activation of a p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide. J. Immunol. 156:4867-4875.
26 Nathan, C. and M.U. Shiloh. 2000. Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens. PNASU. 97:8841-8848.   DOI