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http://dx.doi.org/10.7732/kjpr.2020.33.6.696

Isolation of New Strain of Cordyceps militaris HB8 and Optimal Condition for Production of Adenosine and Cordycepin in Fruit Body  

Li, Jin Feng (Department of Oriental Medicinal Biotechnology, Kyung Hee University)
Hoang, Van An (HanbangBio Inc. Kyung Hee University Holdings)
Ahn, Jong Chan (Graduate School of Biotechnology, Kyung Hee University)
Yang, Dong Uk (HanbangBio Inc. Kyung Hee University Holdings)
Lee, Dong Wook (HanbangBio Inc. Kyung Hee University Holdings)
Yang, Deok Chun (HanbangBio Inc. Kyung Hee University Holdings)
Publication Information
Korean Journal of Plant Resources / v.33, no.6, 2020 , pp. 696-706 More about this Journal
Abstract
Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 mg/g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 mg/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).
Keywords
Adenosine; Cordycepin; Cordyceps militaris; Fruiting-body;
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1 Ahn, H.Y., J.H. Lee, M.J. Kang, J.Y, Cha and Y.S. Cho. 2012. Fibrinolytic activity and chemical properties of cordycepinenriched Cordyceps militaris JLM 0636. J. Life Sci. 22(2):226-231 (in Korean).   DOI
2 Cai, Z.L., C.Y. Wang, Z.J. Jiang, H.H. Li, W.X. Liu, L.W. Gong, P. Xiao and C.H. Li. 2013. Effects of cordycepin on Y-maze learning task in mice. Eur. J. Pharmacol. 714:249-253.   DOI
3 Tuli, H.S., A.K. Sharma, S.S. Sandhu and D. Kashyap. 2013. Cordycepin: A bioactive metabolite with therapeutic potential. Life Sci. 93(23):863-869.   DOI
4 Yang, H., S. Kim, M. Jang, H. Kim, S. Lee, Y. Kim, Y. Eom, G. Kang, L. Chiang, J.H. Baek, J.H. Ryu, Y.E. Lee, J. Koh and H. Jung. 2019. Two-phase delivery using a horse oil and adenosine-loaded dissolving microneedle patch for skin barrier restoration, moisturization, and wrinkle improvement. J. Cosmet. Dermatol. 18(3):936-943.   DOI
5 Zhang, J, C. Wen, Y. Duan, H. Zhang and H. Ma. 2019. Advance in Cordyceps militaris (Linn) Link polysaccharides: Isolation, structure, and bioactivities: A review. Int J Biol Macromol. 132:906-914.   DOI
6 Choi, Y.S., H.K. Kim, B.J. Lee and Y.G. Kim. 2009. Characteristics and breeding of a new variety Cordyceps militaris Yedang 3. J. Mushroom Sci. Prod. 7(4):182-186 (in Korean).
7 Cha, W.S., B.S. Cho and S.Y. Park. 2004. A study on the composition of Cordyceps militaris extract and mycelium. J. Life Sci. 14(5):727-731 (in Korean).   DOI
8 Chin, J.E., H.S. Lee and K.C. Kim. 2006. Effect of mushroom extracts on tyrosinase promoter. Korean J. Sanitation 21(3):1-8 (in Korean).
9 Choi, I.Y., J.S. Choi, W.H. Lee, Y.J. Yu, G.T. Joung, I.O. Ju and T.K. Choi. 1999. The condition of production of artificial fruiting body of Cordyceps militaris. Korean J. Mycol. 27(4):243-248 (in Korean).
10 Choi, J.H., G.S. Kim, S.E. Lee, J.E. Cho, G.H. Sung, D.Y. Lee, S.Y. Kim, T.H. Lee and H.J. Noh. 2012. Anti-inflammatory effects of Cordyceps militaris extracts. J. Mushroom Sci. Prod. 10(4):249-253.
11 Das, S.K., M. Masuda, A. Sakurai and M. Sakakibara. 2010. Medicinal uses of the mushroom Cordyceps militaris: Current state and prospect. Fitoterapia 81(8):961-968.   DOI
12 Dong, J.Z., C. Lei, X.R. Ai and Y. Wang. 2012. Selenium enrichment on Cordyceps militaris Link and analysis on its main active components. Appl. Biochem. Biotechnol. 166:1215-1224.   DOI
13 Hall, T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Window 95/98/NT. Nucleic Acids Symposium, Oxford University Press, Oxford, UK. pp. 95-98.
14 Jo, S.J., T.H. Lee, D.H. Chae and Y.H. Han. 2004. Optimization of culture condition and media composition on the production of cordycepin by Cordyceps militaris. Korean J. Microbiol. 40(3):217-220 (in Korean).
15 Hong, S.M., H.D. Cho, J.H. Kim, J.Y. Lee, J.M. Park and K.I. Seo. 2016. Anti-oxidant and anti-proliferative effects of water extract mixture of Cordyceps militaris and Allium tuberosum. J. Life Sci. 26:805-811 (in Korean).   DOI
16 Hur, H. 2008. Chemical ingredients of Cordyceps militaris. Mycobiology 36:233-235.   DOI
17 Jeong, J.W., C.Y. Jin, M. O. Kim, J.Y. Lee, Y.H. Choi and J.D. Lee. 2009. RAPD analysis and cordycepin concentration of hybrided Cordyceps militaris strains by mating. Korean J. Mycol. 37(1):86-90 (in Korean).   DOI
18 Kim, H.J., T.H. Lee, Y.S. Kwon, M.W. Son and C.K. Kim. 2012. Immunomodulatory activities of ethanol extract of Cordyceps militaris in immunocompromised mice. J. Korean Soc. Food Sci. Nutr. 41(4):494-500 (in Korean).   DOI
19 Jo, S.J., T.H. Lee, D.H. Chae and Y.H. Han. 2005. Effect of light conditions on production of cordycepin of Cordyceps militaris. Korean J. Microbiol. 41(3):236-238 (in Korean).
20 Kang, N., H.H. Lee, I. Park and Y.S. Seo. 2017. Development of high cordycepin-producing Cordyceps militaris strains. Mycobiology 45(1):31-38.   DOI
21 Kim, H.S., Y.J. Rho and M. Choe. 2005. Cordyceps militaris increases hepatic glucokinase activities. J. Korean Soc. Food Sci. Nutr. 34(2):158-161 (in Korean).   DOI
22 Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press, Cambridge, UK.
23 Kim, J.R. S.H. Yeon, H.S. Kim and Y.J. Ahn. 2002. Larvicidal activity against Plutella xylostella of cordycepin from the fruiting body of Cordyceps militaris. Pest Manag. Sci. 58(7):713-717.   DOI
24 Kim, S.W., C.P. Xu, H.J. Hwang, J.W. Choi, C.W. Kim and J.W. Yun. 2003. Production and characterization of exopolysaccharides from an entomopathogenic fungus Cordyceps militaris NG3. Biotechnol. Prog. 19(2):428-435.   DOI
25 Kim, Y.D., S.H. Kwak, K.J. Kim, K.S. Seo, T.Y. Park, K.Y. Yu and S.W. Jin. 2014. The analysis of useful components in Flammulina velutipes fruit body, Flammulina velutipes mycelium and Cordyceps militaris mycelium. J. Mushroom 12(3):193-200.   DOI
26 Lee, B.J., M.A. Lee, Y.G. Kim, K.W. Lee, Y.S. Choi and B.E. Lee. 2015. Varietal characteristics of cross-bred Cordyceps militaris 'Dowonhonghco'. J. Mushroom Sci. Prod. 13(3):151-156.   DOI
27 Lee, S.M., N.S. Park and E.J. Park. 2006. Effects of Cordyceps militaris cultivated on rice on lipid metabolism in rats fed high fat-cholesterol diet. J. Food Sci. Nutr. 11:36-41 (in Korean).
28 Lee, D.E., S.Y. Lee, J.S. Kim, C.K. Cho, H.S. Yoo and S.I. Choi. 2009. Antitumor effect of Hang-Am-Dan (HAD) and its ingredients on Calu6 and MCF-7 human cancer cell lines. J. Korean Oriental Med. 30(5):50-60 (in Korean).
29 Lee, H.M., Y.J. Lee and T.S. Park. 2004. Tumor growth inhibitory and immunomodulatory activities of Cordyceps militaris water extracts in ICR mice bearing Sarcoma-180 solid tumor. J. Korean Soc. Food. Sci. Nutr. 33:59-65 (in Korean).   DOI
30 Lee, S.M, Y.G. Kim, H.C. Park, K.K. Kim, H.J. Son, C.O. Hong and N.S. Park. 2017. Properties of the silkworm (Bombyx mori) Dongchunghacho, a newly developed Korean medicinal insect-borne mushroom: Mass-production and pharmacological actions. Korean J. Life Sci. 27(2):247-266 (in Korean).   DOI
31 Lou, H., J. Lin, L. Guo, X. Wang, S. Tian, C. Liu, Y. Zhao and R. Zhao. 2019. Advances in research on Cordyceps militaris degeneration. Appl. Microbiol. Biotechnol. 103(19):7835-7841.   DOI
32 Masino, S.A., M. Jr. Kawamura, L.M. Plotkin, J. Svedova, Fj Jr. DiMario and I.M. Eigsti. 2011. The relationship between the neuromodulator adenosine and behavioral symptoms of autism. Neurosci Lett. 500(1):1-5.   DOI
33 Nallathamby, N., G.S. Lee, S. Vidyadaran, S.N. Abd Malek, J. Raman and V. Sabaratnam. 2015. Ergosterol of Cordyceps militaris attenuates LPS induced inflammation in BV2 Microglia cells. Nat. Prod. Commun. 10(6):885-886.
34 Lee, B.J., M.A. Lee, Y.G. Kim, K.W. Lee, Y.S. Choi and B.E. Lee. and H.Y. Song. 2013. Cultural characteristics of Cordyceps militaris strain 'Yedang 3' on various media and nutritional conditions. J. Mushroom Sci. Prod. 11(3):124-130.   DOI
35 Seo, K.H., J.Y. Park, H.J. Noh, J.Y. Lee, E.Y. Young, J.G. Han, J.H. Kim and M.S. Cheong. 2018. Anti-inflammatory effects of various mushrooms in LPS-stimulated RAW264.7 cells. Korean J. Plant Res. 31(5):478-488 (in Korean).   DOI
36 Ohta, Y., J.B. Lee, K. Hayashi, A. Fujita, D.K. Park and T. Hayashi. 2007. In vivo anti-influenza virus activity of animmunomodulatory acidic polysaccharide isolated from Cordyceps militaris grown on germinated soybeans. J. Agric. Food Chem. 55:10194-10199.   DOI
37 Park, C.S., C.J. Kwon, M.A. Choi, G.S. Park and K.H. Choi. 2002. Antioxidative and nitrite scavenging activities of Cordyceps militaris extracts. Korean J. Food Preserv. 9:109-113 (in Korean).
38 Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4(4):406-425.
39 Shrestha, B., W. Zhang, Y. Zhang and X. Liu. 2012. The medicinal fungus Cordyceps militaris: Research and development. Mycol. Prog. 11:599-614.   DOI
40 Shrestha, B., Y.J. Park, S.K. Han, S.K. Choi and J.M. Sung. 2004. Instability in in vitro fruiting of Cordyceps militaris. J. Mushroom Sci. Prod. 2(3):140-144.
41 Sung, G.H., N.L. Hywel-Jones, J.M. Sung, J.J. Luangsa-Ard, B. Shrestha and J.W. Spatafora. 2007. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud. Mycol. 57:5-59.   DOI
42 Sung, J.M., S.H. Kim, C.S. Yoon, G.H. Sung and Y.W. Kim. 1999. Analysis of genetic relationship of Cordyceps militaris in Korea by random amplified polymorphic DNA. Korean J. Mycol. 27(4):256-273 (in Korean).
43 Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28(10):2731-2739.   DOI
44 Tran, N.Q. and D.X. Tran. 2019. Xanthine oxidase inhibitory potential, antioxidant and antibacterial activities of Cordyceps militaris (L.) link fruiting body. Medicines 6(1):1-13   DOI
45 Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Res. 25(24):4876-4882.   DOI