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http://dx.doi.org/10.7732/kjpr.2019.32.5.433

Anti-inflammatory and Anti-Atopic Effects of Crude Extracts and Solvent Fractions of Phormium tenax leaf  

Yang, Kwon Min (Biodiversity Research Institute, Jeju Technopark)
Song, Sang mok (Dermapro Company)
Lee, Doseung (Biodiversity Research Institute, Jeju Technopark)
Yoon, Weon-Jong (Biodiversity Research Institute, Jeju Technopark)
Kim, Chan-Shick (Faculty of Biotechnology, Jeju National University)
Kim, Chang Sook (Faculty of Biotechnology, Jeju National University)
Publication Information
Korean Journal of Plant Resources / v.32, no.5, 2019 , pp. 433-441 More about this Journal
Abstract
This study describes a preliminary evaluation of the anti-inflammatory activity and anti-atopic activity of Phormium tenax leaf extracts. P. tenax leaf was extracted using 70% ethanol and then fractionated sequentially with n-hexane, methylene chloride, ethyl acetate, n-butanol. In order to effectively screen for anti-inflammatory agents, we first investigated the inhibitory effects of P. tenax leaf crude extracts and solvent fractions on production of pro-inflammatory factors[nitric oxide(NO), prostaglandin $E_2(PGE_2)$, inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2)] and pro-inflammatory cytokines [tumor necrosis $factor-{\alpha}(TNF-{\alpha})$, interleukin-6(IL-6) and $interleukin-1{\beta}(IL-1{\beta})$] in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells. In addition, we also evaluated of their inhibitory effect on the atopic dermatitis-like inflammatory markers such as macrophage-derived chemokine(MDC) and thymus and activation-regulated chemokine(TARC) in HaCaT cells. Among the five solvent fractions of P. tenax, methylene chloride and ethyl acetate fractions inhibited production of pro-inflammatory factors and pro-inflammatory cytokines in a dose dependent manner, respectively. These fractions were also showed inhibitory activity for MDC and TARC expression levels in $IFN-{\gamma}-stimulated$ HaCaT cells, respectively. These results suggest that P. tenax have significantly effects of anti-inflammatory activity and anti-atopic activity that might be beneficial for the topical treatment of inflammatory skin disorders.
Keywords
Anti-atopic effect; Anti-inflammatory effect; Phormium tenax;
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