Browse > Article
http://dx.doi.org/10.7732/kjpr.2019.32.4.282

Protective Effect of Water Extract of Leonurus japonicus Houttuyn against HCl/EtOH-induced Gastric Mucosal Damage and Genotoxicity Evaluation using Micronucleus Test  

Nho, Jong Hyun (National Institute for Korean Medicine Development)
Lee, Hyun Joo (National Institute for Korean Medicine Development)
Jang, Ji Hun (National Institute for Korean Medicine Development)
Yang, Beo Dul (National Institute for Korean Medicine Development)
Woo, Kyeong Wan (National Institute for Korean Medicine Development)
Kim, A Hyeon (National Institute for Korean Medicine Development)
Seo, Jae Wan (National Institute for Korean Medicine Development)
Kim, Sun Young (National Institute for Korean Medicine Development)
Cho, Hyun Woo (National Institute for Korean Medicine Development)
Jung, Ho Kyung (National Institute for Korean Medicine Development)
Publication Information
Korean Journal of Plant Resources / v.32, no.4, 2019 , pp. 282-289 More about this Journal
Abstract
Gastritis is an inflammatory disease involving the stomach and is caused by several factors, including stress, non-steroidal anti-inflammatory drugs such as aspirin, liquor, and Helicobacter pylori. In Korea, Leonurus japonicus Houttuyn (LJW) has been used as traditional medicine for vaginal bleeding, hematuria, and bruise. Previous studies have reported that LJW exhibited hepatoprotective, cardioprotective, and anti-hyperlipidemic effect. However, the effect of the water extract of LJW on gastritis was not elucidated. Thus, we evaluated the anti-gastric effect and genotoxicity of LJW. LJW effectively prevented the degeneration of surface mucous cells and glandular epithelial cells and vascular congestion induced by HCl/EtOH. Micronucleus assay indicated that the rate of micronucleated polychromatic erythrocytes/polychromatic erythrocytes was not significantly different compared that of the control. Further experiments are required to determine the role of LJW in the gastric injury process such as cyclooxygenase signaling pathway and the secretion of mucus in the stomach.
Keywords
Gastiritis; Genotoxicity; Leonurus japonicus Houttuyn;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Alrashdi, A.S., S.M. Slalma, S.S. Alkiyumi, M.A. Abdulla, A.H. Hadi, S.I. Abdelwahab, M.M. Taha and N. Asykin. 2012. Mechanisms of gastroprotective effects of ethanolic leaf extract of Jasminum sambac against HCl/ethanolinduced gastric mucosal injury in rats. Evid. based Complement Alternat. Med. 2012:786426.
2 Andrews, F.M., C.R. Reinemeyer, M.D. Mccracken, J.T. Blackford, J.A. Nadeau, L. Saabye, M. Sotell and A. Saxton. 2002. comparison of endoscopic, necropsy and histology scoring of equine gastric ulcers. Equine. Vet. J. 34:475-478.
3 Chamberlain, C.E. 1993. Acute hemorrhagic gastritis. Gastroenterol. Clin. North Am. 22:843-873.   DOI
4 Chen, T., W. Jiang, H. Zhang, X. You, M. Liu, L. Wang, P. Xiang, L. Xu, D. Zheng, X. Zhang, H. Ji, K. Hao and T. Yan. 2016. Protective effect of trillin against ethanol-induced acute gastric lesions in an animal model. RSC. Adv. 6:20081-20088.   DOI
5 Choi, W.S., D.Y. Jang, G.R. Lee and S.H Kim. 2012. Protective effect of HEAC and its primary active compound on HClethanol induced gastritis in rats. J. Korea Acad. Industr. Coop. Soc. 13:1769-1776.   DOI
6 Corvi, R.S., Albertini, T. Hartung, S. Hoffmann, D. Maurici, S. Pfuhler, J. Benthem and P. Vanparys. 2008. ECVAM retro spective validation of in vitro micronucleus test (MNT). Mutagen 23:271-283.   DOI
7 Gao, L., M.N. Weck, C. Stegmaier, D. Rothenbacher and H. Brenner. 2009. Alcohol consumption and chronic atrophic gastritis: population-based study among 9,444 older adults from Germany. Int. J. Cancer 125:2918-2922.   DOI
8 Hoshino, T., S. Tsutsumi, W. Tomisato, H.J. Hwang, T. Tsuchiya and T. Mizushima. 2003. Prostaglandin $E_2$ protects gastric muscosal cells from apoptosis via $EP_2$ and $EP_4$ receptor activation. J. Biol. Chem. 287:12752-12758.
9 Hwang, I.Y. and C.S Jeong. 2013. A study on the antigastiritic effect of Rheum species extracts and their active components. J. Fd. Hyg. Safety 28:330-336.   DOI
10 Hwang, I.Y., S.Y. Lee, S.Y. Kyung and C.S. Jeong. 2016. Inhibitory effects of Polygala tenuifolia Willd., Polygala japonica and Polygala tenuifolia on HCl.ethanol-induced gastritis in rats. Yakhak Hoeji. 61:24-29.   DOI
11 Jeon, W.Y., M.Y. Lee, I.S. Shin, S.E. Jin and H. Ha. 2015. Curcuma aromatica water extract attenuates ethanol-induced gastritis via enhancement of antioxidant status. Evid. Based Complement Alternat. Med. 2015:582496.
12 Jin, S.E., M.Y. Lee, I.S Shin, W.Y. Jeon and H. Ha. 2016. Syzygium aromaticum water extract attenuates ethanol-induced gastric injury through antioxidant effects in rats. Mol. Med. Rep. 14:361-366.   DOI
13 Jo, Y.K. 2013. The development of anti-wrinkle cosmeceutical ingredients from Leonuri herba extract. Korean J. Food Preserv. 20:592-596.   DOI
14 Jung, Y.H., Y.M. Ham, S.A. Yoon, D.J. Oh, C.S. Kim and W.J. Yoon. 2018. Antioxidant and anti-inflammatory effects of Ficus erecta var. sieboldii leaf extract in murine macrophage RAW 264.7 cells. Korean J. Plant Res. 31:303-311.   DOI
15 Kandulski, A., M. Selgrad and P. Malfertheiner. 2008. Helicobacter pylori infection: a clinical overview. Dig. Liver Dis. 40:619-626.   DOI
16 Khan, S.O. Shehzad, H.G. Jin, E.R. Woo, S.S. Kang, S.W. Baek, J. Kim and Y.S. Kim. 2012. Anti-inflammatory mechanism of 15, 16-Epoxy-3${\alpha}$-hydroxylabda-8, 13(16), 14-trien-7-one via inhibition of LPS-induced multicellular signaling pathways. J. Nat. Prod. 75:61-71.
17 Kim, H.N., G.H. Park, J.D. Kim, S.B. Park, H.J. Eo and J.B. Jeong. 2019. Effect of the extracts from the leaves and branches of Sageretia thea on ${\beta}$-catenin proteasomal degradation in human colorectal and lung cancer cells. Korean J. Plant Res. 32:153-159.   DOI
18 Kruger, N. J. 2009. The bradford method for protein quantitation. The Protein Protocols Handbook. Springer, Berlin, Germany. pp. 17-24.
19 Kim, S.J., H.S. Han and Y.J. Lee. 2010. Effects of Leonuri herba and Leonuri semen on hypercholesterolemia. Korean J. Herbology 25:73-80.
20 Kim, T.Y., S.A. Jang, Y.B. Chae and J.P. Bak. 2016. Antioxidant and protective effects of Leonurus sibiricus L. extract on ultraviolet B (UVB)-induced damage in human keratinocytes. Korean J. Plant Res. 29:11-19.   DOI
21 Lee, J.Y., O.J. Kwon, J.S Noh and S.S Roh. 2016. Protective effect of red ginseng according to steaming time on HCl/ethanol-induced acute gastritis. J. Appl. Biol. Chem. 59:365-372.   DOI
22 Li, Y.X., Z. Chen, Z.M. Feng, Y.N Yang, J.S. Jiang and P.C. Zhang. 2012. Hepatoprotective glycosides from Leonurus japonicus Houtt. Carbohydr. Res. 348:42-46.   DOI
23 Li, Z., C. Wu, L. Li, Z. Wang, H. Xie, X. He and J. Feng. 2017. Effect of long-term proton pump inhibitor administration on gastric mucosal atrophy: a meta analysis. Saudi J. Gastroenterol. 23:222-228.   DOI
24 Min, B.S. 2012. 13-week repeated-dose toxicity test of Leonuri Herba. KFDA. TRKO201300028832.
25 Nho, J.H., J.H. Jang, H.J. Lee, B.D. Yang, K.W. Woo, A.H. Kim, J.W. Seo, T.Y. Hwang, H.W. Cho and H.K. Jung. 2019. Preventive effect of the water extract of Agrimonia pilosa Ledeb and micronucleus assay-based evaluation of genotoxicity in gastritis animal models. Korean J. Medicinal Crop Sci. 27:136-142.   DOI
26 Park, H.J. and H.S. Kim. 2011. Effects of proton pump inhibitors on atrophic gastritis and gastric cancer: safe perspective. Korean J. Med. 81:1-5.
27 Ricciotti, E. and G.A. Fitzgerald. 2011. Prostaglandins and inflammation. Arterioscler. Thromb. Vasc. Biol. 31:986-1000.   DOI
28 Park, S. K.B. Hahm, T.Y. Oh, J.H. Jin and R. Choue. 2004. Preventive effect of the flavonoid, wogonin, against ethanolinduced gastric mucosal damage in rats. Dig. Dis. Sci. 49:384-394.   DOI
29 Park, S.B., H.M. Song, H.N. Kim, G.H. Park, H.J. Son, Y. Um, J.A. Park and J.B. Jeong. 2018. Anti-inflammatory effect of Biji (Soybean curd residue) on LPS-stimulated RAW264.7 cells. Korean J. Plant Res. 31:117-123.   DOI
30 Park, S.B., H.N. Kim, J.D. Kim, G.H. Park, H.J. Eo, M.Y. An and J.B. Jeong. 2019. Inhibitory effect of the branch extracts from Taxillus yadoriki parasitic to Neolitsea sericea against the cell proliferation in human lung cancer cells, A549. Korean J. Plant Res. 32:109-115.   DOI
31 Sipponen, P and H.I. Maaroos. 2015. Chronic gastritis. Scand. J. Gastroenterol. 50:657-667.   DOI
32 Takeuchi, K. and K. Amagase. 2017. Roles of prostaglandin E and EP receptors in mucosal protection and ulcer healing in the gastrointestinal tract. Arch. Dig. Disord. 1:8-16.
33 Tanigawa, T., T. Watanabe, M. Hamaguchi, E. Sasaki, K. Tominaga, Y. Fujiwara, N. Oshitani, T. Matsumoto, K. Higuchi and T. Arakawa. 2004. Anti-inflammatory effect of two isoforms of COX in H. pylori-induced gastritis in mice: possible involvement of PGE2. Am. J. Physiol. Gastrointest. Liver Physiol. 286:148-156.   DOI
34 Wallace, J.L. 1992. Prostaglandins, NSAIDs, and cytoprotection. Gastroenterol. Clin. North Am. 21:631-641.   DOI
35 Woo, H.S., S.M. Lee, J.D. Heo, M.S. Lee, Y.S. Kim and D.W. Kim. 2018. Anti-inflammatory activity of extracts of Hovenia dulcis on lipopolysaccharides-stimulated RAW264.7 cells. Korean J. Plant Res. 31:466-477.   DOI
36 Yoo, S.R., H. Ha, M.Y. Lee, H.K. Shin, S.C. Han and C.S. Seo. 2017. A 4-week repeated-dose oral toxicity study of Bojungikgitang in Crl:CD Sprague dawley rats. Evid. Based Complement Alternat. Med. 4748904.
37 Yeung, H.W., Y.C Kong, W.P. Lay and K.F. Cheng. 1997. The structure and biological effect of leonurine. Planta Med. 31:51-56.   DOI