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http://dx.doi.org/10.7732/kjpr.2019.32.3.207

Inhibitory Effects of a Combination of Grapefruit and Rosemary Extracts for Alleviating UV-Induced Skin Ageing  

Choi, Hee-Jeong (School of Food Science & Biotechnology, Kyungpook National University)
Alam, Badrul (School of Food Science & Biotechnology, Kyungpook National University)
Zhao, Peijun (School of Food Science & Biotechnology, Kyungpook National University)
Cha, Yeong-Ho (School of Food Science & Biotechnology, Kyungpook National University)
Kim, Tae-Ho (Biomedical Research Institute, Kyungpook National University Hospital)
Lee, Sang-Han (School of Food Science & Biotechnology, Kyungpook National University)
Publication Information
Korean Journal of Plant Resources / v.32, no.3, 2019 , pp. 207-219 More about this Journal
Abstract
Ultraviolet (UV) radiation is associated with the development of extrinsic skin aging. We performed in vivo assays in order to investigate the protective effect of a combination of grapefruit and rosemary extracts (cG&Re) on UVB-induced skin aging. The results indicated that cG&Re displayed elastase inhibitory activity in a dose-dependent manner. Topical application of cG&Re mitigated photo-aging related lesions such as skin erythema and thickening in photo-aged BALB/c mice dorsal skin, by preventing UVB-induced collagen degradation. Immunohistochemical analyses revealed that cG&Re stimulated SIRT-1 expression, and suppressed MMP-1 and $IL-1{\beta}$ expression. It was observed that expression of MMP-1 and -13 mRNA was downregulated in the cG&Re-treated group. Furthermore, cG&Re treatment drastically suppressed protein expression of MMP-1 and regulated the phosphorylation of p-38 kinase. As expected, oral administration of cG&Re resulted in the same SIRT-1, MMP-1, and $IL-1{\beta}$ expression patterns observed upon topical application of cG&Re in the UV-induced mice model. Overall, the current results demonstrated that cG&Re attenuated both the downregulation of MMP-1 expression and up-regulation of SIRT-1 expression, as well as decreased phosphorylation of MAPK in UVB-induced skin ageing mice model, suggesting that cG&Re might be used as an internal food ingredient for beauty-purposes as well as a functional food material.
Keywords
Combination of grapefruit and rosemary (cG&Re); MAPKs; MMP-1; Photo-ageing;
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