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http://dx.doi.org/10.7732/kjpr.2018.31.2.095

Role of Iridin Isolated from Iris koreana Nakai on Doxorubicin-induced Necrosis in HK-2 Cells, and Effect on Cancer Cells  

Nho, Jong Hyun (National Development Institute of Korean Medicine)
Lee, Ki Ho (National Development Institute of Korean Medicine)
Jung, Ho Kyung (National Development Institute of Korean Medicine)
Lee, Mu Jin (National Development Institute of Korean Medicine)
Jang, Ji Hun (National Development Institute of Korean Medicine)
Sim, Mi Ok (National Development Institute of Korean Medicine)
Jung, Ja Kyun (National Development Institute of Korean Medicine)
Jung, Da Eun (National Development Institute of Korean Medicine)
Cho, Hyun Woo (National Development Institute of Korean Medicine)
Publication Information
Korean Journal of Plant Resources / v.31, no.2, 2018 , pp. 95-101 More about this Journal
Abstract
Doxorubicin is a anti-cancer drugs that interferes with the growth and spread of cancer cells in human body. Doxorubicin is used to treat different types of cancers that affect the ovary, thyoid and lungs, but induced side effect such as nephrotoxicity and cardiotoxicity. Thus, we investigated that the effect of iridin on doxorubicin-induced necrosis in HK-2 cells, a human proximal tubule cell. To confirm effect of iridin on doxorubicin-induced necrosis, HK-2 cells are treated with $10{\mu}M$ doxorubicin and $80{\mu}M$ iridin. $80{\mu}M$ iridin reduced $10{\mu}M$ doxorubicin-induced necrosis, the mitochondrial over activation and caspase-3 activation. However, iridin reduces anti-cancer effect of doxorubicin such as PARP1 and caspase-3 activation, checkpoint proteins (CDK4 and CDK6) in NCI-H1129 cells (Human non-small cell lung cancer cell). In HCT-116 cells (Human colorectan cancer cell), iridin do not increased protein expression of CDK4 and CDK6 decreased by doxorubicin. Results indicate that treatment of iridin was diminished doxorubicin-induced necrosis in HK-2 cells. However, iridin was decreased anti-cancer effect of doxorubicin on NCI-H1229, but not HCT-116. Thus, further experiment are required to iridin treatment on various cancer cells and animal models because effect of iridin different cell type.
Keywords
Doxorubicin; HK-2; Iridin; Iris koreana Nakai; Necrosis; Nephrotoxicity;
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