Browse > Article
http://dx.doi.org/10.7732/kjpr.2017.30.4.364

Seed Germination, Plant Growth and Antioxidant Capacity of Limonium tetragonum under Different Salt Concentrations  

Jeong, Jae-Hyeok (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
Hwang, Woon-Ha (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
An, Sung-Hyun (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
Jeong, Han-Yong (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
Lee, Hyeon-Seok (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
Baek, Jung-Sun (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
Choi, Kyung-Jin (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
Lee, Geon-Hwi (Crop Production and Physiology Research Division, National Institute of Crop Science, Rural Development Administration)
Ra, Ji-Eun (Crop Foundation Division, National Institute of Crop Science, Rural Development Administration)
Chung, Nam-Jin (Department of Crop Science & Biotechnology, Chonbuk National University)
Lee, Seung Jae (Department of Chemistry and Research Institute of Physics and Chemistry, Chonbuk National University)
Yun, Song Joong (Department of Crop Science & Biotechnology, Chonbuk National University)
Publication Information
Korean Journal of Plant Resources / v.30, no.4, 2017 , pp. 364-371 More about this Journal
Abstract
Limonium tetragonum is a halophyte grown naturally in the coastal region in South Korea. This study was conducted to investigate the effects of salt concentrations on seed germination, seedling growth, and antioxidant capacity of L. tetragonum. Seeds were collected from naturally grown plants of L. tetragonum and those at full maturity were used in this experiment. All experiments were performed at 0%, 0.5%, 1.0%, or 2.0% of salt concentrations. Seed germination rate was highest as 86% at $20^{\circ}C$ and followed as higher in order of $25^{\circ}C$, $30^{\circ}C$ and $15^{\circ}C$. The germination rate was about 80% at 0% or 0.5% of salt concentration, but it was very low at the salt concentrations higher than 1%. Growth of L. tetragonum seedlings showed no difference in Hoagland solution containing NaCl in the range of 0% to 1.0% and seedlings survived at 2.0% of NaCl concentration. As the salt concentration increased, the content of $Na^+$ in the shoot increased, but that of $K^+$, $Ca^{{+}{+}}$, or $Mg^{{+}{+}}$ decreased. The antioxidant activity and the content of total polyphenol and total flavonoid in the shoot were similar at 0% and 0.5% of NaCl and were highest at 2.0% of NaCl concentration. In conclusion, performance of seed germination and plant growth of L. tetragonum was highest at 0% and 0.5% of NaCl concentration, and showed no difference in antioxidant activity, total polyphenol contents, and total flavonoid contents at the same salt concentrations.
Keywords
Antioxidant capacity; Germination; Growth; Halophyte; Limonium tetragonum;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 McKinstry, P.J., H.E. Indyk and N.D. Kim. 1999. The determination of major and minor elements in milk and infant formula by slurry nebulisation and inductively coupled plasma - optical emission spectrometry (ICP-OES). Food Chem. 65(2):245-252.   DOI
2 Meot-Duros, L., G.L. Floch and C. Magne. 2008. Radical scavenging, antioxidant and antimicrobial activities of halophytic species. J. Ethnopharmacol. 116:258-262.   DOI
3 Moller, J.K.S., H.L. Madsen, T. Altonen and L.H. Skibsted. 1999. Dittany (Origanum dictamnus) as a source of water extractable antioxidants. Food Chem. 64:215-219.   DOI
4 National Institute of Crop Science (NICS). 2014. Analytical Methods of Food Crops and Enviroments. Rural Development Administration (RDA), Suwon, Korea. pp. 78-86.
5 Ra, J.E., J.Y. Park, W.D. Seo, E.Y. Sim, J.Y. Ko, M.H. Nam, I.M. Chung and S.I. Han. 2014. Antioxidative and anti-inflammatory activity of extract from milling by-products of Sorghum cultivar, 'Hwanggeumchal'. Korean J. Crop Sci. 59(4):463-469.   DOI
6 Shim, H.B., S.M. Seo and B.H. Choi. 2002. Floristic survey of salt marshes and dunes on Gyeonggi bay in Korea. Korean J. Enviro. Biol. 20(1):25-34.
7 Sreenivasulu, N., B. Grimm, U. Wobus and W. Weschke. 2000. Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica). Physiol. Plant. 109:435-442.   DOI
8 Stankovic, M.S., M. Petrovic, D. Godjevac and Z. D. Stevanovic. 2015. Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants? J. Arid Environ. 120:26-32.   DOI
9 Ungar, I.A. 1974. The effect of salinity and temperature on seed germination and growth of Hordeum jubatum L. Can. J. Bot. 52:1357-1362.   DOI
10 Ungar, I.A. 1978. Halophyte seed germination. Bot. Rev. 44(2):233-264.   DOI
11 Ungar, I.A. 1996. Effect of salinity on seed germination, growth, and ion accumulation of Atriplex patula (Chenopodiaceae). Am. J. Bot. 83(5):604-607.   DOI
12 Zhu, J.K. 2000. Genetic analysis of plant tolerance using Arabidopsis. Plant Physiol. 124:941-948.   DOI
13 Waisel, Y. 1972. Biology of Halophytes. Academic Press, New York, USA. pp. 212-215.
14 Yang, M.H., N.H. Kim, J.D. Heo, S.H. Sung and E.J. Jeong. 2014. Hepatoprotective effects of Limonium tetragonum, edible medicinal halophyte growing near seashores. Pharmacogn. Mag. 10 (Suppl 3):S563-S568.   DOI
15 Yildiz, M., S. Cenkci and M. Kargioglu. 2008. Effects of salinity, temperature, and light on seed germination in two Turkish endemic halophytes, Limonium iconicum and L. lilacinum (Plumbaginaceae). Seed Sci. Technol. 36:646-656.   DOI
16 Min, B.M. 1998. Vegetation on the west coast of Korea. Ocean Polar Res. 20:167-178.
17 Dietz, B.M., Y.H. Kang, G. Liu, A.L. Eggler, P. Yao, L.R. Chadwick, G.F. Pauli, N.R. Farnsworth, A.D. Mesecar, R.B. van Breemen and J.L. Bolton. 2005. Xanthohumol isolated from Humulus lupulus inhibits menadione-induced DNA damage through induction of quinone reductase. Chem. Res. Toxicol. 18:1296-1305.   DOI
18 Amor, N.B., A. Jimenez, W. Megdiche, M. Lundqvist, F. Sevilla and C. Abdelly. 2006. Response of antioxidant systems to NaCl stress in the halophyte Cakile maritima. Physiol. Plant. 126:446-457.   DOI
19 Bae, M.J., F. Karadeniz, S.G. Lee, Y.W. Seo and C.S. Kong. 2016. Inhibition of MMP-2 and MMP-9 activities by Limonium tetragonum extract. Prev. Nutr. Food Sci. 21(1):38-43.   DOI
20 Dewanto, V., X. Wu, K.K. Adom and R.H. Liu. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem. 50(10):3010-3014.   DOI
21 Erdei, L. and P.J.C. Kuiper. 1979. The effect of salinity on growth, action content, Na+-uptake and translocation in salt-sensitive and salt-tolerant Plantago species. Plant Physiol. 47:95-99.   DOI
22 Heim, K.E., A.R. Tagliaferro and D.J. Bobilya. 2002. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J. Nutri. Biochem. 13:572-584.   DOI
23 Jeong, J.H., T.K. Kim, W.Y. Choi, N.H. Baek, C.H. Yang, D.H. Kim, S. Kim, Y.D. Kim, S.B. Lee, K.B. Lee, K.H. Park and K.M. Cho. 2013. Optimum salinity concentration an nitrogen fertilization for Salicornia herbaecea growth in reclaimed land. Korean J. Intl. Agri. 25(1):62-67.   DOI
24 Isabelle, M.B., L. Lee, C.N. Ong, X. Liu and D. Huang. 2008. Peroxyl radical scavenging capacity, polyphenolics, and lipophilic antioxidant profiles of mulberry fruits cultivated in Southern China. J. Agric. Food Chem. 56:9410-9416.   DOI
25 Jeon, B.Y. and D.H. Park. 2011. Effect of glasswort (Salicornia herbacea L.) and rice (Oryza sativa L.) on quality of Ganjang (Korean soy sauce). Food Sci. Biotech. 20(4):979-986.   DOI
26 Jeong, J.H., S. Kim, J.H. Lee, W.Y. Choi, K.B. Lee and K.M. Cho. 2014. Germination and growth response of Spergularia marina Griseb by salt concentration. Korean J. Crop Sci. 59(2):139-143.   DOI
27 Kim, C.S. and T.G. Song. 1983. Ecological studies on the halophyte communities at western and southern coasts in Korea. Korean J. Ecology 6(3):167-176.
28 Kim, N.H., J.D. Heo, T.B. Kim, J.R. Rho, M.H. Yang and E.J. Jeong. 2015. The extract of Limonium tetragonum protected liver against acute alcohol toxicity by enhancing ethanol eetabolism and antioxidant enzyme activities. Nat. Prod. Sci. 21(1):54-58.
29 Kim, N.H., J.D. Heo, T.B. Kim, J.R. Rho, M.H. Yang and E.J. Jeong. 2016. Protective effects of ethyl acetate soluble fraction of Limonium tetragonum on diethylnitrosamine-induced liver fibrosis in rats. Biol. Pharm. Bull. 39:1022-1028.   DOI
30 Kim, S.S. and H.C. Cha. 2017. Comparison of the total phenolic and flavonoid contents and antioxidant activities of four kinds of sand dune plants living in Taean, Korea. Korean J. Plant Res. 30(1):8-16.   DOI
31 Lee, B.M., S.I. Shim, S.G. Lee, B.H. Kang, I.M. Chung and K.H. Kim. 1999. Physiological response on saline tolerance between halophytes and glycophytes. Korean J. Environ. Agric. 18(1):61-65.
32 Kong, C.S., Y.R. Um, J.I. Lee, Y.A. Kim, J.S. Lee and Y.W. Seo. 2008. Inhibition effects of extracts and its solvent fractions isolated from Limonuim tetragonum of human cancer cells. Korean J. Biotechnol. Bioeng. 23(2):177-182.
33 Ksouri, R., W. Megdiche, H. Falleh, N. Trabelsi, M. Boulaaba, A. Smaoui and C. Abdelly. 2008. Influence of biological, environmental and technical factors on phenolic content and antioxidant activities of Tunisian halophytes. C. R. Biologies 331:865-873.   DOI
34 Kwon, M.S., F. Karadeniz, S.G. Lee, Y.W. Seo and C.S. Kong. 2016. Adipogenesis inhibitory effects of Limonium tetragonum in mouse bone marrow stromal D1 cells. Food Sci. Biotechnol. 25(S):125-131.
35 Lee, H.J., Y.A. Kim, J.W. Ahn, B.J. Lee, S.G. Moon and Y. Seo. 2004. Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants. Korean J. Biotechnol. Bioeng. 19:57-61.
36 Lee, J.I., C.S. Kong, M.E. Jung, J.W. Hong, S.Y. Lim and Y.W. Seo. 2011. Antioxidant activity of the halophyte Limonium tetragonum and its major active components. Biotech. Bioprocess Eng. 16:992-999.   DOI
37 Madsen, H.L., B.R. Nielsen, G. Bertelsen and L.H. Skibsted. 1996. Screen of antioxidative activity of spices. A comparison between assays based on ESR spin trapping and electrochemical measurement of oxygen consumption. Food Chem. 57:331-337.   DOI