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http://dx.doi.org/10.7732/kjpr.2015.28.5.635

Triglyceride Control Effect of Agrimonia eupatoria L. in Oleic Acid Induced NAFLD-HepG2 Model  

Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
Kim, Taeseong (Department of Herbal Medicine Resource, Kangwon National University)
Jeong, Yong Joon (Department of Life Science, Gachon University)
Han, Hyo-Sang (Department of Health Administration, Joongbu University, Geumsan 32713, Korea)
Lea, Youngsung (BIOKOREA CO., LTD.)
Cho, Young Mi (Department of Life Science, Gachon University)
Kang, Se Chan (Department of Life Science, Gachon University)
Publication Information
Korean Journal of Plant Resources / v.28, no.5, 2015 , pp. 635-640 More about this Journal
Abstract
Nonalcoholic fatty liver disease (NAFLD) is a kind of liver inflammation caused by an accumulation of fat in the liver. Patients with NAFLD have an increased risk to develop liver fibrosis, which leads to cirrhosis. To investigate hepatoprotective effects of Agrimonia eupatoria L (A. eupatoria), oleic acid-induced NAFLD in HepG2 cells was used and A. eupatoria was fractionated with ethanol (EtOH), n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH), and H2O. Cells treated with the EtOAc fraction showed the highest lipid accumulation inhibiting effect. A. eupatoria also suppressed triglyceride accumulation and inhibited expression of lipid marker gene, such as a peroxisome proliferator activated receptor γ (PPAR-γ). Moreover, another marker, mRNA expression level of peroxisome proliferator activated receptor α (PPAR-α) was significantly increased by in a dose-dependent manner. These results suggest that A. eupatoria is a potent agent for the treatment of NAFLD.
Keywords
NAFLD; Agrimonia eupatoria; PPAR-γ ; Oleic acid; HepG2;
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