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http://dx.doi.org/10.7732/kjpr.2015.28.4.526

Production of Baicalin, Baicalein, and Wogonin on Plant Tissue Culture of Scutellaria baicalensis  

Hwang, In-Taek (Department of Medical Biotechnology, Soonchunhyang University)
Lee, Jae-Jin (Department of Medical Biotechnology, Soonchunhyang University)
Lee, Joo–Young (Department of Medical Biotechnology, Soonchunhyang University)
Paik, Seung-Woo (Crop Resource Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Kim, Yong-Ho (Department of Medical Biotechnology, Soonchunhyang University)
Publication Information
Korean Journal of Plant Resources / v.28, no.4, 2015 , pp. 526-532 More about this Journal
Abstract
Scutellaria baicalensis Georgi (SJ) is a perennial plant and its root has been used in oriental traditional medicine for treatment of fever, inflammation, diarrhea and anticancer effect, etc. In this study, plant tissue culture system for SJ was developed. Stem piece of younger plant was optimum explant for callus induction and growth on MS medium supplemented with NAA 1.0 ㎎/L plus BA 0.5 ㎎/L. Plantlet regeneration through callus culture was well on MS medium containing NAA 1.0 mg/L. SJ has been known biologically active substances such as baicalin, baiclein, and wogonin. This study was carried out to examine the effect of plant growth regulators for production of baicalin, baicalein, and wogonin through callus culture. The HPLC pattern of callus extract was identical to that of standard solution, it shows that the callus produced by tissue culture has the same flavonoids composition of SJ. Baicalin, baicalein, and wogonin production was 471.5~52.8 ㎍/g, 137.6~4.0 ㎍/g, and 16.6~1.3 ㎍/g, respectively, on MS media with nine different plant growth regulator combinations. This may indicate that plant tissue culture of SJ possible to produce the biologically active substances effectively
Keywords
Scutellaria baicalensis Georgi; Tissue culture; HPLC; Baicalin; Baicalein; Wogonin;
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1 Zheng, J., J.D. Hu, Y.Y. Chen, B.Y. Chen, Y. Huang, Z.H. Zheng and T.B. Liu. 2012. Baicalin induces apoptosis in leukemia HL-60/ADR cells via possible down-regulation of the PI3K/Akt signaling pathway. Asian Pacific J. Cancer Prev. 13(4):1119-1124.   DOI
2 Zhou, B.R., W.I. Liu and D. Luo. 2011. Protective effect of baicalin against multiple ultraviolet B exposure-mediated injuries in C57BL/6 mouse skin. Arch. Pharm. Res. 34(2): 261-268.   DOI
3 Kim, S.J., S.J. Kim, K.M. Kim and S.M. Lee. 2012. Protective effect of baicalin against Hepatic ischemia/Reperfusion injury in alcoholic fatty liver. Yakhak Hoeji 56(4):260-267 (in Korean).
4 Lee, M.S., K.H. Kim and O.K. Hong. 1993. Plant regeneration from leaf tissue culture and some effective substances in Scutellaria baicalensis G. Korean J. Medicinal Crop Sci. 1(1):43-48 (in Korean).
5 Kim, Y.H., D.W. Jeong, Y.C. Kim, D.H. Sohn, E.S. Park and H.S. Lee. 2007. Pharmacokinetics of baicalein, baicalin and wogonin after oral administration of a standardized extract of Scutellaria baicalensis, PF-2405 in rats. Arch. Pharm. Res. 30(2):260-265.   DOI
6 Lee, H.J. 2005. Inhibition effect of Scutellaria baicalensis Georgi extracts on osteoclast differentiation and bone resorption. MS Thesis, Keimyung University, Korea (in Korean).
7 Lee, J.K. 2008. Analgesic and anti-inflammatory effect of Scutellaria baicalensis. MS Thesis, Kyungwon Univ., Korea (in Korean).
8 Piao, H.Z., S.A. Jin, H.S. Chun, J.C. Lee and W.K. Kim. 2004. Neuroprotective effect of wogonin: Potential roles of inflammatory cytokines. Arch. Pharm. Res. 27(9):930-936.   DOI
9 Seo, O.N., G.S. Kim, Y.H. Kim, S. Park, S.W. Jeong, S.J. Lee, J.S. Jin and S.C. Shin. 2013. Determination of polyphenol components of Korean Scutellaria baicalensis Georgi using liquid chromatography-tandem mass spectrometry: Contri- bution to overall antioxidant activity. J. Functional Foods 1741-1750.
10 Seo, W.T. 1995. 5(4):Production of flavone glycosides in cell suspension culture of Scutellaria baicalensis Georgi. Ph.D Thesis, Kon-Kuk Univ., Korea (in Korean).
11 Shin, S.W. and H.K. Lee. 1995. Production of baicalin by cell culture of Scutellaria baicalensis. Korean J. Pharmacogn. 26(2):159-163 (in Korean).
12 Heller, W and G. Forkmann. 1988. Biosynthesis: In The Flavonoids, Harbone, J.B. (ed.), Chapman & Hall, London, UK. pp. 339-425.
13 Bae, J.H., M.J. Lee and S.M. Lee. 2005. Antimicrobial effect of Scutellaria baicalensis Georgi extracts on food-borne pathogens. Korean J. Microbio. Biotech. 33(1):35-40 (in Korean).
14 Kim, H.J., S.Y. Kim, Y.S. Lee and Y.H. Kim. 2014. Determination of baicalin and baicalein contents in Scutellaria baicalensis by NIRS. Korean J. Plant Res. 27(4):286-292 (in Korean).   DOI
15 Choi, M.R., J.S. Lee and H.S. Lim. 2007. Changes in physiological activities of Scutellaria Baicalensis by heating. J. Life Sci. 17(10):1381-1386 (in Korean).   DOI
16 Gang, M.H., J.H. Lee, Y.S. Lee, K.H. Son, D.H. Lee, Y.S. Kim, S.S. Kang, H.C. Bang and C.S. Jeong. 2007. Antigastritis and anti Helicobacter pylori effects of baicalein from Scutellaria Baicalensis. Yakhak Hoeji 51(1):68-74 (in Korean).
17 Ikemoto, S., K. Sugimura, N. Yoshida, R. Yasumoto, S. Wada, K. Yamamoto and T. Kishimoto. 2000. Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines. Urology 55(6):951-955.   DOI
18 Kim, J.H. 1998. Comparative studies on change of flavonoid composition and pharmacological activity of Scutellaria baicalensis by processing. Ph.D. Thesis, Sookmyung Women's Univ., Korea (in Korean).