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http://dx.doi.org/10.7732/kjpr.2014.27.6.650

Identification and Quantification of Flavonoids in Korean Wild Grape (Meoru grape, Vitis coignetiae) and Its Pomace  

Shim, Kwan-Seob (Department of Animal Biotechnology, Chonbuk National University)
Kang, Da-Rae (Department of Animal Biotechnology, Chonbuk National University)
Park, Seong-Bok (National Institute of Animal Science, RDA)
Park, Jong Hyuk (Institute of Natural Medicine, Hallym University)
Chung, Yi Hyung (Jeonbuk Institute for Bioindustry)
Kang, Young-Hee (Department of Food and Nutrition, Hallym University)
Shin, Daekeun (Department of Food and Nutrition, Hallym University)
Publication Information
Korean Journal of Plant Resources / v.27, no.6, 2014 , pp. 650-659 More about this Journal
Abstract
Large quantities of Korean wild grape (Vitis coignetiae, KWG) pomace, a by-product of grape juice and wine manufacture, are generated annually, and disposal cost of KWG pomace is now increasing. Therefore, this study was conducted to determine total polyphenol, flavonoid, and anthocyanin content in whole KWG, KWG skin and KWG pomace and to identify and quantify flavonoids found in whole KWG and KWG pomace using LC/MS/MS. Spectral analysis showed high total polyphenol and flavonoid in KWG skin extracted with 75% ethanol (p < 0.05). KWG pomace had higher amount of total polyphenol, flavonoid and anthocyanin than whole grape (p < 0.05). Sixteen flavonoids were identified, but only 10 flavonoids were quantifiable from whole KWG and KWG pomace. Both epicatechin and rutin were the major flavonoids, and 521 or 147 ng/g of epicatechin (p < 0.05) and 305 or 110 ng/g of rutin (p > 0.05) were found in whole KWG and KWG pomace, respectively. The results show that KWG pomace is a very rich source of flavonoids, thus KWG pomace can be used as a functional food additive. Plans to include KWG pomace in food production are necessary.
Keywords
By-product; Ethanol; Korean wild grape skin; LC/MS/MS; Polyphenol;
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