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http://dx.doi.org/10.7732/kjpr.2014.27.5.439

Anti-inflammatory Effect of Castanopsis cuspidata Extracts in Murine Macrophage RAW 264.7 Cells  

Ko, Yeong-Jong (Biodiversity Research Institute, Jeju TECHNOPARK)
Song, Sang Mok (Biodiversity Research Institute, Jeju TECHNOPARK)
Hyun, Woo-Chol (Biodiversity Research Institute, Jeju TECHNOPARK)
Yang, Soo-Kyung (Biodiversity Research Institute, Jeju TECHNOPARK)
Song, Chang-Khil (Faculty of Biotechnology, Jeju National University)
Lee, Dong-Sun (Major of Plant Resource & Environment, Jeju National University)
Yoon, Weon-Jong (Biodiversity Research Institute, Jeju TECHNOPARK)
Publication Information
Korean Journal of Plant Resources / v.27, no.5, 2014 , pp. 439-446 More about this Journal
Abstract
This study describes a preliminary evaluation of the anti-inflammatory activity of Castanopsis cuspidata extracts. C. cuspidata was extracted using 80% ethanol and then fractionated sequentially with n-hexane, dichloromethane, ethylacetate, and butanol. To screen for anti-inflammatory agents effectively, we first examined the inhibitory effect of the C. cuspidata extracts on the production of pro-inflammatory factors and cytokines stimulated with lipopolysaccharide. In addition, we examined the inhibitory effect of C. cuspidata extracts on pro-inflammatory mediators (NO, iNOS, COX-2) in murine macrophage RAW 264.7 cells. The amounts of protein levels were determined by immunoblotting. Of the sequential solvent fractions of C. cuspidata, the n-hexane, dichloromethane and ethylacetate fractions inhibited the mRNA expression of pro-inflammatory cytokines (IL-$1{\beta}$ and IL-6), production of NO, and the protein level of iNOS and COX-2. These results suggest that C. cuspidata may have significant effects on inflammatory factors and may be provided as a possible anti-inflammatory therapeutic plant.
Keywords
Castanopsis cuspidata; Pro-inflammatory factors; Cytokines; Lipopolysaccharide; RAW 264.7 cells;
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