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http://dx.doi.org/10.7732/kjpr.2013.26.5.658

The Optimal Combination of the Mixture of Unripe Rubus coreanus and Astragalus membranaceus in the Activation and Differentiation of Osteoblastic Cells  

Koo, Hyun Jung (Department of Life Science, Gachon University)
Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
Kang, Se-Chan (Department of Life Science, Gachon University)
Publication Information
Korean Journal of Plant Resources / v.26, no.5, 2013 , pp. 658-662 More about this Journal
Abstract
Rubus coreanus and Astragalus membranaceus Bunge have been used as a traditional medicine with various pharmacological properties for a long time in Asia. We investigated the proliferative and differentiative effects on osteoblastic cells, MG-63 in various mixture ratios of immature fructus extracts of R. coreanus and root extracts of A. membranaceus. With preliminary in vivo ovariectomized mice, we confirmed the effects of the supplementation of various mixture on alkaline phosphatase (ALP) activity in serum. The ratio of 7:3 is considered as the most effective ratio for the proliferation of osteoblastic cells as they increased by 56.8% similar to estradiol. ALP activity in osteoblast increased by the combination of R. coreanus and A. membranaceus and peaked in the ratio of 72:28 and 68:32 respectively. These results indicate that the optimal combination of R. coreanus and A. membranaceus in 7:3 has the most beneficial effects on the activation of osteoblast cells, and give further possibilities that it can be used clinically in the treatment of osteoporosis.
Keywords
Alkaline phosphatase; Astragalus membranaceus; MG-63; Osteocalcin; Rubus coreanus;
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