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http://dx.doi.org/10.7732/kjpr.2013.26.4.417

Improving Effects of Platycodon Extracts Jelly on β-amyloid-induced Cytotoxicity and Scopolamine-Induced Cognitive Impairment Animal Models  

Oh, Hong-Geun (Huvet Co. Ltd.)
Kim, Jung-Hoon (Huvet Co. Ltd.)
Shin, Eun-Hye (Huvet Co. Ltd.)
Kang, Young-Rye (Huvet Co. Ltd.)
Lee, Bong-Gun (Huvet Co. Ltd.)
Park, Sang-Hoon (Huvet Co. Ltd.)
Moon, Dae-In (Huvet Co. Ltd.)
Kwon, Lee-Seong (Namyoung Pharm)
Kim, Yong-Phill (Namyoung Pharm)
Choi, Min-Hyu (Namyoung Pharm)
Kim, Ok-Jin (Animal Disease Research Unit, Wonkwang University)
Park, Kwang-Hyun (Department of Oriental Pharmaceutical Development, Nambu University)
Lee, Hak-Yong (Huvet Co. Ltd.)
Publication Information
Korean Journal of Plant Resources / v.26, no.4, 2013 , pp. 417-425 More about this Journal
Abstract
The aim of this study was to examine improving effect of Platycodon extracts (PE) and/or Platycodon extracts jelly (PEJ) on cognitive impairment in vitro and in vivo. PC12 (Pheochromocytoma) cells were pretreated with PE for 1hr and than incubated with $50{\mu}M$ amyloid ${\beta}(A{\beta})_{25-35}$ for additional 48hr. Cell viability was assessed by WST-1. Animals for Morris water test and passive avoidance test were divided into normal, control and two Platycodon extracts treated groups that were named Normal (n=7), Control (0 mg/kg, n=7), PE (300 mg/kg, n=7), PEJ (10 g/kg, n=7). Cognitive impairment was induced by scopolamine (1 mg/kg/body weight, i.p.) in the three experimental groups but not the normal group. Pretretment of PE (0.01-1 mg/mL) were not induced cytotoxicity but observed in high dose-treated group (5 and 10 mg/mL) in PC12 cells. Protective effects of PE against $A{\beta}$-induced cytotoxicity were increased in dose dependent manner in PC12 cells. Administration of PE and PEJ were significantly reduced escape latency time on Morris water maze test and passive avoidance test in copolamine-induced cognitive impairment animal model. These results suggest that Platycodon extracts and its related product available to ameliorative purpose for cognitive ability impairments.
Keywords
Platycodon; Cognitive ability; Amyloid-${\beta}$; Scopolamine; PC12 cells;
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