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http://dx.doi.org/10.7732/kjpr.2013.26.2.310

Genetic Diversity and Structure of the Korean Endemic Species, Coreanomecon hylomeconoides Nakai, as Revealed by ISSR markers  

Son, Sung-Won (Plant Conservation Division, Korea National Arboretum)
Chung, Jae-Min (Plant Conservation Division, Korea National Arboretum)
Kim, Eun-Hye (Plant Conservation Division, Korea National Arboretum)
Choi, Kyoung-Su (Department of Life Science, Yeungnam University)
Park, SeonJoo (Department of Life Science, Yeungnam University)
Publication Information
Korean Journal of Plant Resources / v.26, no.2, 2013 , pp. 310-319 More about this Journal
Abstract
The genetic diversity and structure of eight populations of Coreanomecon hylomeconoides Nakai, an endemic Korean plant, were investigated using 50 ISSR loci from eight primers. The average percentage of polymorphic loci was 47.3%. The Shannon's index (SI=0.218) and gene diversity (h=0.142) were relatively lower than those of other long-lived perennials. The Sancheong (SI=0.233, h=0153), Gwangyang (SI=0.263, h=0.171), and Suncheon (SI=0.241, h=0.159) populations showed greater genetic diversity than the Namhae and Gwangju populations, which are on the edge of the distribution. Analysis of molecular variance (AMOVA) showed that 18% of the total variation could be attributed to differences among populations, and 82% to differences within populations, indicating moderate gene flow among adjacent populations. These results were supported by value of Nm (2.184). The UPGMA conducted using the genetic distance and Bayesian cluster analysis showed a remarkable geographic trend structured into east and west regions. Overall, the results indicate that the Sancheong and Gwangyang populations, which had a large population size and higher degree of genetic diversity, should be the focus of in situ conservation.
Keywords
Coreanomecon hylomeconoides; Genetic diversity; Endemic plant; In situ conservation; ISSR;
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