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http://dx.doi.org/10.7732/kjpr.2012.25.5.561

Study on the Immunomodulatory Effects of Ellagic Acid and their Mechanisms Related to Toll-like Receptor 4 in Macrophages  

NamKoong, Seung (Department of Physical Therapy, Kangwon National University)
Kim, Ye-Jin (Department of Herbal Medicine Resource, Kangwon National University)
Kim, Taeseong (Department of Herbal Medicine Resource, Kangwon National University)
Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.25, no.5, 2012 , pp. 561-567 More about this Journal
Abstract
Ellagic acid (EA) is a phenolic compound in fruits and nuts including raspberries, strawberries, grapes and walnuts. Previous studies have indicated that EA possesses antioxidant activity, growth-inhibition and apoptosis-promoting activity in cancer cells. However, macrophage mediated cytotoxicity and immunomodulating effects on cancer cells have not been clarified. In the present study, we show that EA increased effects on macrophage mediated tumoricidal activity and NO production without direct tumor cell cytotoxicity. To further determine whether TLR4 (toll like receptor 4) is involved in anti-tumor activity, cells were treated TLR4 signaling inhibitor, CLI-095 in the presence of EA. CLI-095 treatment partially reduced macrophage-mediated tumoridial activity. EA also has inhibitory effects of NO production induced by LPS, whereas phagocytic activity was not changed. These results suggest that EA induces macrophage mediated tumoricidal activity which is partially related to TLR4 signaling and has a potential adjuvant in cancer therapy.
Keywords
Anti-tumor activity; Ellagic acid; Macrophage; Nitric oxide; Toll like receptor 4;
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