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http://dx.doi.org/10.7732/kjpr.2011.24.5.557

Anti-tumor Activity of Saponin Fraction of Platycodon gradiflourm through Immunomodulatory Effects associated with NO production in RAW264.7 cells  

Choung, Myoung-Gun (Department of Herbal Medicine Resource, Kangwon National University)
Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.24, no.5, 2011 , pp. 557-563 More about this Journal
Abstract
Platycodon grandiflorum (Korean name, Doraji) has been widely used in traditional herbal medicine as an expectorant for pulmonary disease and a remedy for respiratory disorders in Asia. Here, we investigated the effects of BtOH extract saponin fraction of P. gradiflourm (PGS) on phagocytosis and anti-tumor activity with related cytokine productions in RAW264.7 macrophage cells. The results showed that PGS increased phagocytosis, anti-tumor activity, TNF-${\alpha}$ and nitric oxide (NO) production without direct tumor cell cytotoxicity. To further investigate whether NO is involved in anti-tumor and phagocytic activities of PGS, cells were co-treated with specific iNOS inhibitors, L-NIL (N6-(1-iminoethyl)-L-lysine, dihydrochloride), to block NO production. PGS decreased anti-tumor activity in L-NIL-treated cells, whereas phagocytic activity was not inhibited under the same conditions, indicating that the anti-tumor activity by PGS appears to be conducted by NO. These findings suggest that P. grandiflorum could be used a potential nutrition therapeutic agent for cancer patients.
Keywords
Anti-tumor activity; Nitric oxide; Phagocytosis; Platycodon grandiflorum; RAW264.7;
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