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http://dx.doi.org/10.7732/kjpr.2011.24.4.456

The Antioxidative Effects of Rhododendron brachycarpum Extracts  

Rhim, Tae-Jin (Department of Biotechnology in Division of Animal and Life Resources, Sangji University)
Choi, Moo-Young (Department of Food and Nutrition, Sangji University)
Publication Information
Korean Journal of Plant Resources / v.24, no.4, 2011 , pp. 456-460 More about this Journal
Abstract
The objective of this study was to investigate the antioxidative capacity of Rhododendron brachycarpum 95% ethanol extracts. Total antioxidant status was examined by total antioxidant capacity against ABTS radical reactions. Total antioxidant capacities of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 0.33 and 2.26 mM Trolox equivalents, respectively. Superoxide scavenging activities of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 45.0 and 77.0%, respectively. Oxygen radical absorbance capacities of R. brachycarpum extract at the concentrations of 5 and 100 ${\mu}g/mL$ were 40.88 and 131.00 ${\mu}M$ Trolox equivalents, respectively. Total phenolic contents of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 0.37 and 1.25 mM gallic acid equivalents, respectively. R. brachycarpum extract at the concentration of 0.1 mg/mL inhibited 0.2 mM and 0.5 mM tert-butyl hydroperoxide induced cyototoxicity by 52.1 and 30.3%, respectively, in HepG2 cell culture system. Thus, strong antioxidant and cytotoxicity-inhibiting effects of R. brachycarpum extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents.
Keywords
Rhododendron brachycarpum; ABTS radical; Superoxide; ORAC; Cytotoxicity;
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1 Thannickal, V.J. and B.L. Fanburg. 2000. Reactive oxygen species in cell signaling. American J. Physiol. Lung Cell Mol. Physiol. 279:L1005-L1029.
2 Tirmenstein, M.A., F.A. Nicholls-Grzemski, J.-G. Zhang and M.W. Fariss. 2000. Glutathione depletion and the production of reactive oxygen species in isolated hepatocyte suspensions. Chem. Biol. Interact. 127:201-217.   DOI   ScienceOn
3 Huang, D., B. Ou, M. Hampsch-Woodill, J. A. Flanagan and R. L. Prior. 2002. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agric. Food Chem. 50:4437-4444.   DOI   ScienceOn
4 Jung, S.J., D.-H. Kim, Y.-H. Hong, J.-H. Lee, H.-N. Song, Y.-D. Rho and N.-I. Baek. 2007. Flavonoids from the flower of Rhododendron yedoense var. Poukhanense and their antioxidant activities. Arch. Pharm. Res. 30:146-150.   DOI
5 Liu, F., V.E.C. Ooi and S.T. Chang. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 60:763-771.   DOI   ScienceOn
6 Singleton, V.L. and R. Orthofer. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299:152-178.   DOI
7 Halliwell, B., J.M.C. Gutteridge and C.E. Cross. 1992. Free radicals, antioxidants and human disease:where are we now? J. Lab Clin. Med. 119:598-620.
8 Byun, K.S., Y.W. Lee, H.J. Jin, M.K., Lee, H.Y. Lee, K.J. Lee, M.Y. H, C.Y. Yu and J.H. Lee. 2005. Genotoxicity and cytotoxicity in human cancer and normal cell lines of the extracts of Rhododendron brachycarpum D. Don leaves. Korean J. Medicinal Crop Sci. 13:199-205 (in Korean).
9 Choi, J.S., H.S. Young, J.C. Park, J.-H. Choi and W.S. Woo. 1986. Flavonoids from the leaves of Rhododendron brachycarpum. Arch. Pharm. Res. 9:233-36.   DOI   ScienceOn
10 Erel, O. 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 37:277-285.   DOI   ScienceOn
11 Steel, R.G.D. and J.H. Torrie. 1980. Principles and procedures of statistics, 2nd ed, McGraw-Hill, New York, pp. 186-187.
12 Ahn, D.K. 2000. Illustrated book of Korean medicinal herbs. Kyohaksa. Seoul, Korea. p.751 (in Korean).
13 Yang Y.J., H.-J. Kim, S.-H. Kang and S.C. Kang. 2011. Screening of natural herb resources for anti-oxidative effects in Korea. Korean J. Plant Res. 24:1-9 (in Korean).   DOI
14 Youn, H. and J.-H. Cho. 1991. Isolation and structure elucidation of triterpenoidal constituents from the leaves of Rhododendron brachycarpum. Korean J. Pharmacogn. 22:18-21.