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Isolation and Quantitative Analysis of Flavonoids with Peroxynitritescavenging Effect from the Young Leaves of Heracleum moellendorffii  

Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University)
Nugroho, Agung (Department of Agro-industrial Technology, Faculty of Agriculture, Lambung Mangkurat University)
Jung, Bo-Ram (Department of Pharmaceutical Engineering, Sangji University)
Won, Yu-Hwa (Department of Pharmaceutical Engineering, Sangji University)
Jung, Youn-Ju (Department of Pharmaceutical Engineering, Sangji University)
Kim, Won-Bae (Institute of Horticultural & Herbal Science, Rural Development Administration)
Choi, Jae-Sue (Devision of Food Science and Biotechnology, Pukyong National University)
Publication Information
Korean Journal of Plant Resources / v.23, no.5, 2010 , pp. 393-398 More about this Journal
Abstract
Naturally occurring substances which strongly scavenge peroxynitrite(ONOO-) are evaluated to be beneficial for cardiovascular, diabetic, hypercholesterolemic and obese disease. To search for bioactive substances with peroxynitritescavenging activity from the young leaves of Heracleum moellendorffii(Umbelliferae) used for a mountainous vegetable, the experiment on peroxynitrite-scavenging assay, phytochemical isolation and HPLC analysis were undertaken. Activity-guided fractionation led to the separation of astragalin and hyperoside which were identified by physicochemical and spectroscopic data. The $IC_{50}$ values of astragalin and hyperoside were shown as $5.80{\pm}0.62$ and $0.560{\pm}0.26\;{\mu}M$, respectively, in the assay. The HPLC analysis led to the quantitative determination of astragalin and hyperoside by $30.0{\pm}0.01$ and $17.0{\pm}0.02\;mg/g$ dried weight, respectively.
Keywords
Heracleum moellendorffii; Umbelliferae; peroxynitrite; astragalin; hyperin; HPLC;
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