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Toxic Effects of Sodium Alginate from Brown Algae on HepG2 Human Liver Cell Functions  

Kang, Nam-Sung (College of Pharmacy, Sungkyunkwan University)
Pyo, Suhk-Neung (College of Pharmacy, Sungkyunkwan University)
Jung, Da-Hye (College of Pharmacy, Sungkyunkwan University)
Eum, Hyun-Ae (College of Pharmacy, Sungkyunkwan University)
Jang, Ki-Hyo (Department of Food and Nutrition, Kangwon National University)
Um, Byung-Hun (Natural Product Research Center, Korea Institute of Science and Technology KIST Gangneung Institute)
Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.23, no.2, 2010 , pp. 151-156 More about this Journal
Abstract
Alginates are polysaccharides isolated from brown algae with gel-forming properties composed of 1,4-linked beta-D-mannuronic acid (M), alpha-L-guluronic acid (G), and alternating (MG) blocks. In this study, we have examined the toxic effects of high M-alginate to activate HepG2 human liver cells. Alginate enhanced the NO production and iNOS protein expression in HepG2 cells. In addition, alginates stimulated the HepG2 to induce IL-1 release and expression of TGF-beta1, which could influence the liver inflammation and chirrhosis. These findings suggest that high M-alginate form brown algae may have toxic effects on liver cells.
Keywords
sodium alginate; liver cells; TGF-beta; IL-1beta; iNOS;
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1 McClain, C.J., Song, Z., Barve, S.S. and Hill, D.B. and Deaciuc, I. 2004. Recent advances in alcoholic liver disease: dysregulated cytokine metabolism in alcoholic liver disease. Am. J. Physiol. Gastrointest. Liver Physiol. 287:G497-502.   DOI
2 Nagata, K., Suzuki, H., Nagata, K., Suzuki, H., Sakaguchi, S., Sakaguchi, S. 2007. Common pathogenic mechanism in development progression of liver injury caused by non-alcoholic or alcoholic steatohepatitis. J. Toxicol. Sci. 32(5):453-468.   DOI   ScienceOn
3 Blobe, G.C., Schiemann, W.P. and Lodish, H.F. 2000. Role of transforming growth factor-$\beta$ in human disease. N. Engl. J. Med. 342:1350-1358.   DOI   ScienceOn
4 Bradford, M.M. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem. 72:248-254.   DOI   ScienceOn
5 Geller, D.A., Freeswick, P.D., Nguyen, D., Nussler, A.K., Di Silvio, M., Shapiro, R.A., Wang, S.C., Simmons, R.L. and Billiar, T.R. 1994. Differential induction of nitric oxide synthase in hepatocytes during endotoxemia and the acutephase response. Arch. Surg. 129:165-171.   DOI   ScienceOn
6 Hartsfield, C.L. 2002. Cross talk between carbon monoxide and nitric oxide. Antioxid. Redox. Signal. 4:301-307.   DOI   ScienceOn
7 Hierholzer, C., Harbrecht, B., Menezes, J.M., Kane, J., MacMicking, J., Nathan, C.F., Peitzman, A.B., Billiar, T.R., and Tweardy, D.J. 1998. Essential role of induced nitric oxide in the initiation of the inflammatory response after hemorrhagic shock. J. Exp. Med. 187:917-928.   DOI   ScienceOn
8 Otterlei, M., Ostgaard, K., Skjak-Braek, G., Smidsrod, O., Soon-Shiong, P. and Espevik, T. 1991. Induction of cytokine production from human monocytes stimulated by alginate. J. Immunother. 10:286-291.   DOI
9 Pedersen, S.S., Moller, H., Espersen, F., Sorensen, C.H., Jensen, T. and Hoiby, N. 1992. Mucosal immunity to Pseudomonas aeruginosa alginate in cystic fibrosis. APMIS. 100(4):326-334.   DOI
10 Schlictman, D., Kavanaugh-Black, A., Shankar, S. and Chakrabarty, A.M. 1994. Energy metabolism and alginate biosynthesis in Pseudomonas aeruginosa; Role of the tricarboxylic acid cycle. J. Bacteriol. 176(19):6023-6029.   DOI
11 Seljelid, R. 1989. Tumor regression after treatment with aminated $\beta1-3D$ polyglucose is initiated by circulatory failure. Scand. J. Immunol. 29:181-188.   DOI   ScienceOn
12 Soon-Shiong, P., Otterlie, M., Skjak-Braek, G., Smidsrod, O., Heintz, R., Lanza, R.P. and Espevik, T. 1991. An Immunologic basis for the fibrotic reaction to implantation microcapsules. Transplant. Proc. 23:758-759.
13 Iizima-Mizui, N., Fujihara, M., Himeno, J., Komiyama, K., Umezawa, I. and Nagumo, T. 1985. Antitumor activity of polysaccharide fraction from the brown seaweed Sargassum Kjellmanianum. Kitasato. Arch. Exp. Med. 58:59-65.
14 Taylor, B.S., de Vera, M.E., Ganster, R.W., Wang, Q., Shapiro, R.A., Morris Jr., S.M., Billiar, T.R. and Geller, D.A. 1998. Multiple NF-kappaB enhancer elements regulate cytokine induction of the human inducible nitric oxide synthase gene. J. Biol. Chem. 273:15148-15156.   DOI
15 Wang, Y., Vodovotz, Y., Kim, P.K.M., Zamora, R. and Billiar, T.R. 2001. Mechanisms of hepatoprotection by nitric oxide. Ann. N.Y. Acad. Sci. 962:415-422.
16 Zamora, R., Vodovotz, Y. and Billiar, T.R. 2000. Inducible nitric oxide synthase and inflammatory diseases, Mol. Med. 6:347-373.
17 Krough, J., Hoiby, H., Stenvang, N. and Pedersen, S. 1991. Experimental immunization with Pseudomonas aeruginosa alginate induces IgA and IgG antibody response. APMIS. 99(12):1061-1068.   DOI   ScienceOn