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Antioxidative Effect and Melanogenesis of Nelumbo nucifera Stamen Extract on Cultured Human Skin Melanoma Cells Injured by Hydrogen Peroxide  

Kim, Myoung-Seoup (Department of Environmental Health Science, Wonkwang University)
Park, Yun-Jum (Division of Horticulture and Animal-Plant Science, Wonkwang University)
Sohn, Young-Woo (Sanbon Medical Center, Wonkwang University)
Publication Information
Korean Journal of Plant Resources / v.23, no.2, 2010 , pp. 145-150 More about this Journal
Abstract
To examine the antioxidative effect and melanogenesis of Nelumbo nucifera stamen (NNS) extract on hydrogen peroxide $H_2O_2$ induced cytotoxicity in cultured human skin melanoma cells (SK-MEL-3), cell adhesion activity (CAA), tyrosinase inhibitory activity and total amount of melanin synthesis were measured by colorimetric assay. In this study, $H_2O_2$ significantly decreased CAA, and $CAA_{50}$ value of $H_2O_2$ was determined at 30 uM. In the antioxidative effect, NNS extract increased cell adhesion activity which was decreased by $H_2O_2$ induced cytotoxicity, and also, tyrosinase activity and total amount of melanin were decreased by NNS extract. These results suggested that $H_2O_2$ was highly toxic on cultured human skin melanoma cells and NNS extract showed the antioxidative and inhibitory effect of melanogenesis by the increased CAA, and the decresed tyrosinase activity and total amount of melanin synthesis.
Keywords
Tyrosinase activity; Cell adhesion activity; Cytotoxicity; Colorimetric assay; Inhibitory effect; Plant extract;
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