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Direct Multiple Shooting Induction of Taraxacum  

Gou, Xiaoxia (College of Life Sicience, Northeast Forestry University)
Kim, Jae-Hak (Department of Plant Biotechnology, Kangwon National University)
Hong, Soon-Kwan (Department of Plant Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.22, no.6, 2009 , pp. 522-527 More about this Journal
Abstract
Plants of the genus Taraxacum are well-known as a traditional herbal remedy with a long history, and they have also been extensively used as food, chemicals and cosmetics. In this study, four Taraxacum species distributed in Korea (T. mongolicum, T. officinale, T. mongolicum variation and T. officinale variation) were utilized for an efficient method for direct multiple shooting induction and regeneration, using leaf blade, transition zone, petiole and root as explants in MS media with various hormone concentration and combination. MS medium containing IAA 0.2 mg/L and TDZ 1.0 mg/L showed the highest induction frequency of all the hormone combinations. Besides, the induction of T. mongolicum variation was most effective comparing with the other three species by the average induction frequency of four explants. While the induction effect of leaf blade explant was more obvious than the other three explants. This system exhibited a rapid propagation of shoots from the leaf blade explants and makes it convenient to make use of these Taraxacum species to develop their diverse applications in the future.
Keywords
Multiple shoots; Taraxacum; Explants; TDZ; Vitrification; Organogenesis;
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