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Buddleja officinalis prevents the normal cells from oxidative damage via antioxidant activity  

Hong, Se-Chul (School of Bioresources, Andong National University)
Jeong, Jin-Boo (School of Bioresources, Andong National University)
Jeong, Hyung-Jin (School of Bioresources, Andong National University)
Publication Information
Korean Journal of Plant Resources / v.21, no.6, 2008 , pp. 449-456 More about this Journal
Abstract
The flowers of Buddleja officinalis are used to treat sore and damaged eyes, a condition which is similar to skin wounds. However, whether it has any protective effect on oxidative DNA damage and cell death induced by hydroxyl radical remains unclear. In this study, we evaluated the protective effects of the extracts against oxidative DNA and cell damage caused by hydroxyl radical. DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging assay, and $Fe^{2+}$ chelating assay were used to evaluate the antioxidant properties. phi X 174 RF I plasmid DNA and intracellular DNA migration assay were used to evaluate the protective effect against oxidative DNA damage. Lastly, MTT assay and lipid peroxidation assay were used to evaluate the protective effect against oxidative cell damage. It was found to prevent intracellular DNA and the normal cells from oxidative damage caused by hydroxyl radical via antioxidant activities. These results suggest that Buddleja officinalis may exert the inhibitory effect on ROS-induced carcinogenesis by blocking oxidative DNA damage and cell death.
Keywords
Reactive Oxygen Species (ROS); Oxidative DNA damage; Oxidative Cell Death; Carcinogenesis; Lipid peroxidation;
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1 Barreto, R., Kawakita, S., Tsuchiya, J.,Minelli, E., Pavasuthipasit, K., Helmy, A., Marotta, F., 2005. Metal-induced oxidative damage in cultured hepatocytes and hepatic lysosomal fraction: beneficial effect of a curcumin/absinthium compound. Chinese Journal of Digestive Diseases. 6: 31-36   DOI   ScienceOn
2 Chaudhary, A.K., Nokubo, M., Reddy, G.R., Yeola, S.N., Morrow, J.D., Blair, I.A., Marnett, L.J., 1994. Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science. 265; 1580-1582   DOI
3 Esterbauer, H., 1982. In: McBrien, D.C.H., Slater, T.F. (Eds), Free Radicals, Lipid Peroxidation and Cancer. Academic Press, New York, pp 101-128
4 Kang, K.A., Zhang, R., Piao,M.J., Ko, D.O.,Wang, Z.H., Kim, B.J., Park, J.W., Kim, H.S., Kim, D.H., Hyun, J.W., 2008. Protective effect of irisolidone, a metabolite of kakkalide, against hydrogen peroxide induced cell damage via antioxidant effect. Bioorganic & Medicinal Chemistry. 16; 1133-1141   DOI   ScienceOn
5 Li, Y., Trush, M.A., 1993. Oxidation of hydroquinone by copper: chemical mechanism and biological effects. Arch. Biochem. Biophys. 300: 346-355   DOI   ScienceOn
6 Lloyd, R.V., Hanna, P.M.,Mason, R.P., 1997. The origin of the hydroxyl radical oxygen in the Fenton reaction. Free Radic. Biol. and Med. 22: 885-888
7 Smirnoff, N., Cumbes, Q.J., 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry. 28: 1057-1060   DOI   ScienceOn
8 Livingstone, D.R., 2001. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms, Mar. Pollut. Bull. 42: 656-666   DOI   ScienceOn
9 Rosenkranz, A.R., Schmaldienst, S., Stuhlmeier, K.M., Chen, W., Knapp,W., Zlabinger, G.A., 1992.Microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescindiacetate. J. Immunol. Methods. 156: 39-45   DOI   ScienceOn
10 Armitage P., Doll, R., 1954. The age distribution of cancer and amulti-stage theory of carcinogenesis. Br. J. Cancer. 8: 1-12   DOI   ScienceOn
11 Schwarz, S.M., Peres, G., Kunz,W., Furstenberger, G., Kittstein, W.,Marks, F., 1984. On the role of superoxide anion radicals in skin tumour promotion. Carcinogenesis. 5: 1663-1670   DOI   ScienceOn
12 Ghosal, D., Omelchenko,M.V., Gaidamakova, E.K.,Matrosova, V.Y., Vasilenko, A., Venkateswaran, A., Zhai, M., Kostandarithes, H.M., Brim, H., Makarova, K.S., Wackett, L.P., Fredrickson, J.K.,Daly,M.J., 2005. Howradiation kills cells: survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. FEMS Microbiol. Rev. 29: 361-375   DOI   ScienceOn
13 Hus, B., Coupar, I.M., Ng, K., 2006. Antioxidant activity of hot water extract from the fruit of the Doum palm. Hyphaene thebaica. Food Chem. 98: 317-328   DOI   ScienceOn
14 Jung, Y, Surh, Y, 2001. Oxidative DNAdamage and cytotoxicity unduced by copper-stimulated redox cycling of salsolinol, a neurotoxic tetrahydroisoquinoline alkaloid. Free Radic. Biol. Med. 30: 1407-1417   DOI   ScienceOn
15 Grisham,M.B., 1992. Reactive oxygenmetabolism. In: Grisham M.B., ed. Reactive metabolites of oxygen and nitrogen in biology and medicine. Austin: RG Landers Company. pp 39
16 Powell, C.L., Swenberg, J.A., Rusyn, I., 2005. Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage. Cancer Letters. 229: 1-11   DOI   ScienceOn
17 Barja, G., 2004. Free radicals and aging, Trends Neurosci. 27: 595-600   DOI   ScienceOn
18 Wang, H., Gao, X.D., Zhou, G.C., Cai, L., YaO,W.B., 2008. In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris furit. Food Chemistry. 106: 888-895   DOI   ScienceOn
19 Stohs, S.J., Bagchi, D., 1995. Oxidative mechanism in the toxicity of metal ions. Free Radic. Biol. and Med. 18: 321-336   DOI   ScienceOn
20 Houghton, P.J., 1984. Ethnopharmacology of some Buddleja species, Journal of Ethnopharmacology. 11: 293-308   DOI   ScienceOn
21 Leonard, S.S., Keil, D., Mehlman, T., Proper, S., Shi, X., Harris, G.K., 2006. Essiac Tea: Scavenging of reactive oxygen species and effects on DNA damage. Journal of Ethnopharmacology. 103: 288-296   DOI   ScienceOn