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Characteristics of Agronomy Traits to Transgenic Rice Selected by Molecular Breeding Method  

Lee, Hyun-Suk (School of Applied Biosciences, Kyungpook National University)
Kang, Hyun-Goo (Department of Plant Resources, College of Life Science and Natural Resources, Kyungpook National University)
Park, Young-Hie (School of Applied Biosciences, Kyungpook National University)
Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
Kim, Chang-Kil (Department of Environmental Horticulture, College of Life Science and Natural Resources, Kyungpook National University)
Han, Jeung-Sul (National Institute of Hortcultural and Meicinal Crop, Rural Development Administration)
Sohn, Jae-Keun (School of Applied Biosciences, Kyungpook National University)
Kim, Kyung-Min (Department of Environmental Horticulture, College of Life Science and Natural Resources, Kyungpook National University)
Park, Gyu-Hwan (Department of Plant Resources, College of Life Science and Natural Resources, Kyungpook National University)
Publication Information
Korean Journal of Plant Resources / v.21, no.5, 2008 , pp. 388-394 More about this Journal
Abstract
This study was carried out to develop new cultivars using the $T_5$ generation of transformed rice by PCR analysis with DNA marker in each generation $(from\;T_3\;to\;T_5)$. In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis $H^+/Ca^{2+}$ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants $(T_5)$ were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.
Keywords
Rice; Molecular breeding; CAX 1; Disease resistance;
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