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Effective Micropropagation of Pulsatilla cernua var. koreana through Apical Meristem Culture  

Ko, Jeong-Ae (Faculty of Biological Resources Science, College of Agriculture, Chonbuk National University, Institute of Agricultural Science & Technology, Chonbuk National University)
Kim, Hyun-Soon (National Institute of Crop Science, Rural Development Administratiion)
Publication Information
Korean Journal of Plant Resources / v.21, no.5, 2008 , pp. 362-367 More about this Journal
Abstract
In order to investigate the effect of plant growth regulators on effective in vitro micropropagation, apical meristems of Pulsatilla cernua var. koreana were cultured on Murashige and Skoog's (MS) medium with 2,4-D, NAA, TDZ and BA. Media containing 2,4-D and kinetin, 2,4-D and TDZ, NAA and TDZ were not effective on callus induction. However, embryogenic or organogenic callus was obtained on media containing NAA and BA. Especially, on MS medium with 0.5mg/L NAA and 1.0mg/L BA was optimal for a high frequency (62%) of shoot or shoot bud obtained from callus. Callus proliferation, shoot multiplication and elongation were significantly increased by adding 10% coconut water on MS media with 0.5mg/L NAA and 1.0mg/L BA. Repeated subculturing of in vitro grown shoots resulted in propagation rate of 12.9 shoots per explant every 30 days. Root formation from the adventitious shoots was not easily achieved. However, roots were only produced through callus on MS medium with 2.0mg/L NAA alone or 0.5mg/L NAA and 1.0mg/L BA. These roots were used materials for callus and shoot production repetitively.
Keywords
Optimal medium; Coconut water; NAA; BA;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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