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Transformation of Tobacco by Gene-gun  

Kim, Byung-Oh (Department of Applied Biology, Kyungpook National University)
Kim, Kyung-Min (Department of Environmental Horticulture, Kyungpook National University)
Oh, Jung-Youl (Department of Environmental Horticulture, Kyungpook National University)
Publication Information
Korean Journal of Plant Resources / v.21, no.4, 2008 , pp. 249-253 More about this Journal
Abstract
Since there is no report about more than one gene expression simultaneously in a single mitochondria, this report is very important to novel type of eukaryotic gene expression. In this study, investigated whether mitochondrial expressed gene and GFP that expression in mitochondria of plant expressed to mitochondria. Expression vector (pBin) containing AtBI-1 (mitochondrial expressed gene) and GFP driven by 35S promoter was bombarded by gene gun to leaves and cotyledon of tobacco. Regenerated shoot confirmed expression of AtBI-1 in mitochondria by GFP expression, PCR, and Southern analysis. Successful mitochondria of plant cell transformation in this report implies possible eukaryotic mitochondrial transformation including plants and animals, and moreover two or more gene expression which can be excellent applicable protocols to pharmaceutical field including antibody production.
Keywords
Tobacco; Gene-gun; Mitochondria;
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Times Cited By KSCI : 1  (Citation Analysis)
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