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Transformation of 'Ilmibyeo' using pCAMBIA 1300 and Microstructural Investigation of Leaves  

Guo, Jia (School of Biotechnology, Kangwon National University)
Seong, Eun-Soo (School of Biotechnology, Kangwon National University)
Kim, Young-Hwa (Department of Plant Biotechnology, Dongguk University)
Jo, Hye-Jeong (School of Biotechnology, Kangwon National University)
Cho, Joon-Hyeong (Department of Plant Biotechnology, Dongguk University)
Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.20, no.5, 2007 , pp. 437-441 More about this Journal
Abstract
The argE gene of E.coli was introduced into #Ilmibyeo# cultivar of rice by Agrobacterium tumefaciens and a large number of transgenic plants were produced. Embryogenic calli were co-cultivated with A. tumefaciens strain AGL1 carrying the plasmid pCAMBIA1300 containing hygromycin resistance(HygR). Transgenic plants showing in vitro resistance to 50mg/L hygromycin were obtained using a selection procedure. Stable integration of argE and HPT genes into chromosomal DNA was proven by southern blot analysis and PCR analysis of genomic isolated from $T_0$ progenies. The fragments of 650 bp(HPT) were detected in transgenic rice lines. The 230 bp(argE) fragments were showed in agarose gel, and detected fragments were matched with size of argE specific primer. The microscopic feature of leaf on scanning electron microscope(SEM) revealed differences between clear and chalky in shape and arrangement of stoma but did not discriminate.
Keywords
argE; Hygromycin; Ilmibyeo; SEM;
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