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Induction of Valiant of Cyrtomium caryoptideum var. coreanum Nakai by Chemical Mutagenesis In vitro and RAPD Analysis  

Jeong Jin-A (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Lee Cheol-Hee (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Korean Journal of Plant Resources / v.19, no.2, 2006 , pp. 374-380 More about this Journal
Abstract
With the aim of inducing mutation in fern Cyrtomium caryoptideum var. coreanum, rhizome segments of In vitro-grown cultures were treated with chemical mutagens such as EMS, NMU and colchicine. Based on regeneration ratios, sensitivities for each treatments were assessed and also optimum treatment condition of each mutagens was explored. Optimum concentration for EMS treatment was considered to be 20 to 50mM and for NMU 5 to 10mM. NMU was found to be more effective in inducing chlorophyll and morphological variations than EMS. The RAPD were performed to check the genetic modification of phenotypical variants. As a result, polymorphic DNA band patterns between wild type and variants were observed by two 10-mer primers.
Keywords
EMS; NMU; Colchicine; RAPD;
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