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Intraspecific Relationship Analysis of Safflower (Carthamus tinctorius L.) Lines Collected by RAPD Markers  

Kim Jae-Chul (Research Institute of Natural Product Kyoungbuk Agriculture Technology Administration)
Choi Seong-Yong (Research Institute of Natural Product Kyoungbuk Agriculture Technology Administration)
Shin Dong-Hyun (College of Agriculture and Life Science, Kyungpook National University)
Kim Se-Jong (Research Institute of Natural Product Kyoungbuk Agriculture Technology Administration)
Publication Information
Korean Journal of Plant Resources / v.19, no.2, 2006 , pp. 336-339 More about this Journal
Abstract
This study was conducted to provide the genetic diversity on Safflower collections and to identify the variations which could be utilized in Safflower breeding. The RAPDs analysis was used to clarify the genetic relationships among 32 Safflower collections. Among 37 primers applied in RAPD analysis, 25 primers that generated appropriate PCR products for identification of the genetic characters in safflower collections were used. Amplified PCR showed the highly reproducible bands at $0.1{\sim}4.0kb$. The number of bands amplified in each primer showed the variations ranging from 1 to 9, with the average of 5.6. A total of 25 bands were identified among twenty-five selected primers and 23 bands (19.2%) showed polymorphism. Based on the similarity value of 0.042 in dendrogram derived from the cluster analysis, the 32 Safflower collections were classified into 6 groups. The two main groups, II and III included 12 collections (38%) and 12 collections (38%), respectively.
Keywords
Carthamus tinctorius L.; RAPD; Polymorphism;
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1 Kim, S., Kim, K. Y., Park, M. S., Choi, S. Y. and Yun, S. J. 1998. Intraspecific Relationship of Eleutherococcus senticosus Max. by RAPD Markers. Korean J. Medicinal Crop Sci. 6(3): 165-169
2 Yu K, Pauls KP. 1993. Rapid estimation of genetic relatedness among hetergenous population of affalfa by random amplification of bulked genomic DNA samples. Theor. Appl. Genet. 86: 788-794
3 Williams, J. G. K, A. R. Kubelik, K. H. Livak. J. A. Rafalski and S. V. Fingey. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531-6535   DOI
4 Brown, A. H. D. 1990. The role of isozyme studies in molecular systimatics, Aust. Syst. Bot. 3: 39-46   DOI
5 Bang, K. H., Kim, Y. K., Park, H. W., Seong, N. S., Cho, J. H., Kim, H. S. and Cho Y. G. 2001. Classification of Safflower (Carthamus tinctorius L.) Collections by Agronomic Character istics. Korean J. Medicinal Crop Sci. 9(4): 301-309   과학기술학회마을
6 Echt, C.S, Erdhal and L.A, McCoy. 1992. Genetic Segregation of Random amplified polymorphic DNA in diploid cultivated alfalfa. Genome. 35: 84-87   DOI   ScienceOn
7 Kim, J. S., Choi, S. Y., Choo, B. G., Ryu, J. H., Kwon, T. H. and Oh, D. H. 2000. Intrapecific Relationship of Rehmannia glutinosa Lines Medicinal Collected from Korea, Japan and China by RAPD Analysis. Korean J. Crop Sci. 8(3): 266-273   과학기술학회마을
8 Knowles, P. F. 1989. Safflower in Oil Crops of the World. McGraw-Hill, New York. pp. 363-374