Browse > Article

Several Factors Affecting on In vitro Culture of Prothallus and Ex Vitro Sporophyte Formation from Prothallus of Dyropteris varia (L.) O. Kuntze  

Jeong Jin-A (Dept. of Horticultural Science, Chungbuk National Univ.)
Lee Cheol-Hee (Dept. of Horticultural Science, Chungbuk National Univ.)
Publication Information
Korean Journal of Plant Resources / v.19, no.2, 2006 , pp. 252-258 More about this Journal
Abstract
The most effective conditions of In vitro culture and ex vitro sporophyte formation from prothallus were studied for mass propagation of Dyropteris varia. The most effective medium of prothallus proliferation was Murashige and Skoog's basal medium supplemented with 10:50mM of $NH_4^+:NO_3^-$ and 2% sucrose. The optimum pH level was 5.8 and prothallus growth was promoted on medium containing $0.6{\sim}0.8%$ agar. Almost of the tested growth regulators (NAA, IAA, 2,4-D, BAP, kinetin and 2ip) were inhibitory in prothallus proliferation as the concentration of growth regulators became higher. The highest number of sporophytes was obtained by transplanting prothallus on compost only than on any other soil compositions. Sporophyte formation was promoted remarkably by soaking prothallus with $100{\mu}M\;GA_3$ for 3 hours.
Keywords
Fern; Mass propagation; Medium composition; Soil composition; Priming treatment;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Douglas G.E. and E. Sheffied. 1990. A new technique for the culture of fern gametophytes. Plant Cell Rep. 8: 632-634   DOI   ScienceOn
2 Higuchi, H. and W. Amaki. 1989. Effect of 6-benzylaminopurine on the organogenesis of Asplenium nidis L. through In vitro propagation. Sci. Hort. 37: 351-359   DOI   ScienceOn
3 Jin, Y.H. 1999. Masspropagation of pteridophyta native to Korea by tissue culture. M.S. Thesis, Chungbuk National Univ. pp. 1-62
4 Jones, D.L. 1987. Encyclopedia of ferns. Timer press, Portland
5 Teng, W.L. 1997. Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum. Plant Cell Rep. 17: 77-83   DOI
6 Bertrand, A.M., M.A. Albuerne, H. Fernandez, A. Gonzalez and R. Sanchez-Tames. 1999. In vitro organogenesis of Polypodium cambricum. Plant Cell Tiss. Org. Cult. 57: 65-69   DOI
7 Fernandez, H., A.M. Bertrand and R. Sanchez-Tames. 1997a. Gemmation in Osmunda regalis L. gametophyte cultured In vitro. Plant Cell Rep. 16: 358-362
8 Hvoslef-Eide, A.K. 1991. The effect of temperature, daylength and irradiance on the growth of mother plants of Nephrolepis exaltata (L.) Schott and on the subsequent growth In vitro of runner tip explants. Sci. Hort. 47: 137-147   DOI   ScienceOn
9 Paek, K.Y., C.H. Lee, J.K. Choi and B.H. Kwack. 1984. Masspropagation Nephrolepis exaltata by runner tips In vitro. J. Kor. Soc. Hort. Sci. 25(4): 313-321
10 Salome, M., S. Pais and M. Casal. 1987. Propagation of the fern Adiantum capillus-veneris through tissue culture of the circinate part of young leaves. Acta Hort. (ISHS) 212: 651-654
11 Fernandez, H., A.M. Bertrand, I. Feito and R. Sanchez-Tames. 1997b. Gametophyte culture In vitro and antheridiogen activity in Blechnum spicant. Plant Cell Tiss. Org. Cult. 50(1): 71-74   DOI
12 Padhya, M.A. and A.R. Mehta. 1982. Propagation of fern (Nephrolepis) through tissue culture. Plant Cell Rep. 1: 261-263   DOI   ScienceOn
13 Dawson, L.A., R.W. King and R. van der Staay. 1991. Optimising condition for growth of Nephrolepis ferns. Sci. Hort. 45: 303-314   DOI   ScienceOn
14 Lee, J.S. 2001. Several factors affecting sporophyte formation of three species in pteridophyta. M. S. Thesis, Chungbuk National Univ. pp. 11-13
15 Higuchi, H., W. Amaki and S. Suzuki. 1987. In vitro propagation of Nephrolepis cordifolia Prsel. Sci. Hort. 32: 105-113   DOI   ScienceOn
16 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physical plantarum 15: 473-479   DOI
17 Camloha, M., N. Gogala and J. Rode. 1994. Plant regeneration from leaf explants of the fern Platycerium bifurcatum In vitro. Sci. Hort. 56: 257-266   DOI   ScienceOn
18 Pak, M.K. 1961. Flora of Korean Pteridophyta. Kyohakdoso Co., Seoul
19 Camloha, M. and N. Gogala. 1992. In vitro culture of Platycerium bifurcatum gametophytes. Sci. Hort. 51: 343-346   DOI   ScienceOn