Browse > Article

Molecular Characterization of a cDNA Encoding Chlorophyll a/b Binding Protein (Cab) from Panax ginseng C. A. Meyer  

In Jun Gyo (Research Institute of Biotechnology, Biopia Co., Ltd.)
Lee Bum Soo (Research Institute of Biotechnology, Biopia Co., Ltd.)
Youn Jae-Ho (Sun Chon University)
Son Hwa (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
Kim Se Young (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
Yang Deok Chun (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
Publication Information
Korean Journal of Plant Resources / v.18, no.3, 2005 , pp. 441-449 More about this Journal
Abstract
Photo system II (PSII) is one of the two photosynthetic reaction centers in the chloroplast of higher plants. The chlorophyll a/b-light harvesting complex serves primarily as an antenna for PSII. We isolated a cDNA that encodes a chlorophyll a/b-binding protein (Cab) from Panax ginseng. The small subunit consists of 935 nucleotides long and has an open reading frame of 795 bp with the deduced amino acid of 265 residues (pI 5.63), 28.6 kDa. The deduced amino acid sequence matched to the previously reported Cab genes. Their degree of amino acid identity ranged from 68 to $92\%$. Phylogenetic analysis based on the amino acid residues was showed that the ginseng Cab gene was grouped with P. persica (AAC34983), A. thaliana (AAD28771), G. hirsutum (CAA38025), G. max (AAL29886), and V. radiate (AAF89205).
Keywords
chlorophyll a/b binding proteins; Cab; leaf; Panax ginseng;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Babiychuk, E., R.S. Chantz, N. Cherep, J.H. Weil, Y. Gleband and S. Kushnir. 1995. Alterations in chlorophyll a/b binding proteins in Solanaceae cybrids. Mol. Gen. Genet. 249:648-654   DOI   ScienceOn
2 Morris, P.C., A. Kumar, D.J. Bowles and A.C. Cuming. 1990. Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat. Eur. J. Biochem. 190:625-630   DOI   ScienceOn
3 Millar, A.J. and S.A. Kay. 1991. Circadian control of Cab gene transcription and mRNA accumulation in Arabidopsis. The Plant Cell 3: 541-550   DOI   ScienceOn
4 Park, S.W., S.W. Lee, Y.G. Kand, S.W. Lee, C.H. Lee, J.C. Lee and S.Y. Choi. 2001. Expression of Cab (chlorophyll a/b binding protein) gene in transformed plants. Korean J. Plant Tiss. Cult. 28:41-45
5 Millar, A.J. and S.A. Kay. 1996. Integration of circadian and phototransduction pathways in the network controlling Cab gene transcription in Arabidopsis. Proc. Natl. Acad. Sci. USA 93: 15491-15496
6 Altschul, S.F., W. Gish , W. Miller, E.W. Myers and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410   DOI
7 Butler, W.L. 1978. Energy distribution in the photochemical apparatus of photosynthesis. Annu. Rev. Plant Physiol. 29:345-378   DOI   ScienceOn
8 Thornber, J.P. 1975. Chlorophyll-proteins: light harvesting and reaction center components of plants. Annu. Rev. Plant Physiol. 26: 127-158   DOI   ScienceOn
9 Walling, L., G.N. Drews and R.B. Goldberg. 1988. Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc. Natl. Acad. Sci. USA 83:2123-2127
10 Thompson, J.D., D.G. Higgins and T.J. Gilbson. 1994. CLUST AL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-secific gap penalties and weight matrixchoice. Nucleic Acids Res. 22:4673-4680   DOI   ScienceOn
11 Walling, L.L., Y.C. Chang, D.S. Demmin and F.M. Holzer. 1988. Isolation, characterization and evolutionary relatedness of three members from the soybean multigene family encoding chlorophyll a/b binding proteins. Nuc. Acids Res. 16:10477-10492   DOI   ScienceOn
12 Yang, D.C. 1990. Lipid peroxidation of ginseng thy1akoid membrane. Korean J. Ginseng Sci. 14:135-141
13 Yang, D.C., M.W. Kim, Q. Chae and M.S. Kim. 1989. Effects of active oxygen species (1O2, O2-, H2O2) and scavengers on the chlorophyll bleachin of leafburning disease from Panax ginseng C.A. Meyer. Korean J. Ginseng Sci. 13:98-104
14 Dunsmuir, P. 1985. The petunia chlorophyll a/b binding protein genes: a comparison of Cab genes from different gene families. Nuc. Acids Res. 13:2503-2518   DOI   PUBMED   ScienceOn
15 Yang, D.C., Q. Chae, S.J Lee, Y.H. Kim and Y.H. Kang. 1990. Effects of light and photosynthetic electron transport system on the generation of singlet oxygen (1O2) in ginseng thylakoid membrane. Korean J. Ginseng Sci. 14:57-62
16 Cashmore, A.R. 1984. Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide. Proc. Natl. Acad. Sci. USA 81:2960-2964
17 Thornber, J.P. 1986. In Staehlelin, L.A. and Arnzten, C.J. (eds), Encycl. Plant Physiol. New Series, Vol. 43, Photosynthesis III: Photosynthetic Membranes, Berlin, springer-Verlag.pp. 98-142