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Effective In Vitro Propagation from Pedicel Culture of Hippeastrum hybridum Hort. 'Dazzler'  

Kim Myung Jun (Horticultural Science Major, Faculty of Biological Resources Science, College of Agriculture, Chonbuk National University)
Kim Young Sook (Horticultural Science Major, Faculty of Biological Resources Science, College of Agriculture, Chonbuk National University)
Kim Hyun Soon (Horticultural Science Major, Faculty of Biological Resources Science, College of Agriculture, Chonbuk National University)
Ko Jeong Ae (International Technical Cooperation Center Rural Development Administration)
Publication Information
Korean Journal of Plant Resources / v.18, no.3, 2005 , pp. 382-389 More about this Journal
Abstract
This study was conducted to establish the system of effective in vitro propagation by various explant sources culture of Bippeastrum hybridum Hort, 'Dazzler'. We tested the effects of optimal explant source, plant growth regulators on bulblet formation and plant regeneration. Callus was readily produced on the different tissues excised from floral buds whereas, bulbs and shoots were formed only on pedicel explants as compared with anthers, styles and ovaries. Pedicel is the best optimal explant for in vitro propagation. Two distinct pathways, organogenesis through callus and direct bulblet formation, could be recognized in pedicel culture. Up to the $80-100\%$ of bulblet formation and shoot organogenesis from the pedicel in fifteen days before anthesis were effectively induced by MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA. Plantlet regeneration was successfully achieved from pedicel-derived callus, via shoot bud induction or direct bulblet formation. The bulblets with blooming flower were produced within 2 years.
Keywords
bulblet formation; organogenesis;
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