Browse > Article

Growth and DNA Alteration of Heavy-ion Beam Irradiated Tobacco(Nicotiana plumbaginifolia) Plant  

Lyu Jae-Il (Department of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University)
Kim Min-Su (Department of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University)
Tomoko Abe (Discovery Research Institute, The Institute of Physical and Chemical Research)
Lee Hyo-Yeon (Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University)
Yang Deok-Chun (College of Life Science & Center for Oriental Medicinal Materials and Processing, Kyung Hee University)
Bae Chang-Hyu (Department of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.18, no.1, 2005 , pp. 169-178 More about this Journal
Abstract
Effects of heavy-ion beam$(^{20}Ne)$ irradiation on growth and DNA alteration of tobacco plants were investigated. Seed germination and plant height were decresed as the ion-beam intensity was increased. However, the bolting and flowering were promoted by the low intensities of 5 Gy to 10 Gy treatment. Out of the 100 primers screened, 59 primers generated 336 DNA fragments by RAPD analysis, and one specific DNA fragment that amplified in control but not in the ion-beam irradiated plants was observed. By AFLP analysis, DNA fragment difference related to the ion-beam treatment was not detected but observed among the plant bodys.
Keywords
AFLP; DNA alteration; heavy-ion beam; Nicotiana plumbaginifolia; RAPD;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bae, C.-H., T. Abe, N. Nagata, N. Fukunish, T. Matsuyama, T. Nakano, and S. Yoshida. 2000. Characterization of a periclinal chimera variegated tobacco (Nicotiana tabacum L.). Plant Science 151:93-101   DOI   ScienceOn
2 Vos, P., R. Hoggers, M. Becker, M. Reijans, T. Lee, M. Homes, A. Friejter, J. Pot, J. Peleman, M. Kuiper, and M. Zabeau. 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407-4414   DOI   ScienceOn
3 Li X., Y. Song, K. Century, S. Straight, P. Ronald, X. Dong, M. Lassner, and Y. Zheng. 2001. A fast neutron deletion mutagenesis-based reverse genetics system for plants. Plant J. 27(3):235-242   DOI   ScienceOn
4 Hase, Y., K. Shimono, M. Inoue, A. Tanaka, and H. Watanabe. 1999. Biological effects if ion beams in Nicotiana tabacum L. Radiat. Environ. Biophys. 38:111-115   DOI   ScienceOn
5 Abe, T., C.-H. Bae, T. Ozaki, J.M. Wang, and S. Yoshida. 2000. Stress-tolerant mutants induced by heavy-ion beams. Gamma Field Symposia 39:45-56
6 Chen, Y., B. Jiang, Y. Chen, X. Ding, X. Liu, C. Chen, X. Guo, and G. Yin. 1998. Formation of plasmid DNA strand breaks induced by low-energy ion beam: induction of nuclear stopping effects. Radial. Environ. Biophys. 37:101-106   DOI   ScienceOn
7 Wu, L., and Z. Yu. 2001. Radiobilogical effects of a low-energy ion beam on wheat. Radiat. Environ. Biophys.40:53-57   DOI   ScienceOn
8 WilIams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski, and S.V. Tingey. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535   DOI   ScienceOn
9 배창휴, Abe Tomoko, 민경수, 김동철, 정재성, 이춘환, 임용표, 이효연. 1998. 중이온 beam 조사에 의한 돌연변이 유도와 내염성 식물의 선발. 한국 식물조직배양학회지 25(2):89-94
10 Abe, T., C.-H. Bae, and S. Yoshida. 1997. An effective mutation method for plants using heavy-ion beams. RIKEN Accel. Prog. Rep. 30:127
11 배창휴, Abe Tomoko, 김동철, 이영일, 정재성, 민경수, 이효연. 2000. 중이온 beam 조사가 담배의 약과 캘러스 및 종자에 미치는 영향. 한국식물조직배양학회지. 27:109-115
12 Poresbski, S.L., G. Bailey, and R.B. Baum. 1997. Modification of CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. BioI. Rep. 12:8-15
13 Abe, T., C.-H. Bae, H. Takahashi, and S. Yoshida. 1998. Effective plant-mutation method using heavyion beams(II). RIKEN Accel. Prog. Rep. 31:148
14 Vazquez-Tello, A., T. Uozumi, M. Hidaka, Y. Kobayashi, and H. Watanabe. 1996. Effect of $^{12}C^{+5}$ ion beam irradiation on cell viability and plant regeneration in callus, protoplasts and cell suspensions of Lavatera thuringiaca. Plant Cell Rep. 16:46-49
15 Bae, C.-H., T. Abe, T. Matsuyama, N. Fukunish, N. Nagata, T. Nakano, Y. Kaneko, K. Miyoshi, H. Matsushima, and S Yoshida. 2001. Regulation of chloroplast gene expression is affected in ali, a novel tobacco albino mutant. Annals of Botany 88:545-553   DOI   ScienceOn