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In vitro Culture Response to NaCl of Korean Ginseng (Panax ginseng C.A. Meyer) Tissues  

Yoon Jae-Ho (Institute of KuRae Wild Flower)
Song Won-Seob (College of Agriculture and Life Sciences, Sunchon National University)
Lee Mee Sook (Dept. of Food and Nutrition, Hannam University)
Shin Dong-il (College of Industrial Sciences, Kongju National University)
Yang Deok Chun (College of Life Science, Kyung Hee University)
Publication Information
Korean Journal of Plant Resources / v.18, no.1, 2005 , pp. 123-130 More about this Journal
Abstract
High salt concentrations in the ginseng nursery soil environment of Korea is one of important reducing factors for the stable production of quality ginseng. These studies were accomplished for check the response on germination of ginseng seed, somatic embryogenesis of zygotic embryo, and biosynthesis of ginsenoside from ginseng hairy root against NaCl. Ratio of germination was at the $3\%\;and\;84.5\%$ on the basic media with 0.1M and free of NaCl repectedly, but $0\%$ at the upper of 0.2M NaCl. Somatic embryogenesis from zygotic embryo were the highest when immatured embryo was cultured on free of NaCl concentration, and which was intend to decrease at treatment of NaCl. However, in case of using the matured embryo, treatment of 0.05M NaCl resulted in better embryogenesis than NaCl free media. Red pigment was synthesized from ginseng hairy root cultured on the medium with various NaCl concentration(from 0.04 to 0.08M) and its pigment was analyzed as spectrum of anthocyane by spectrophoto- meter scanning. This cell line biosynthesized lots of crude saponin and total ginsenoside than other cell lines, also had 2 times of panaxadiol than panaxatriol.
Keywords
anthocyane; ginsenoside; germination; hairy root; NaCl; somatic embryogenesis;
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