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Isolation and Characterization of Cyclophilin 1 (ClCyP1) Gene from Codonopsis lanceolata  

양덕춘 (경희대학교 한방재료가공센터)
이강 (㈜바이오피아)
인준교 (㈜바이오피아)
이범수 (㈜바이오피아)
김종학 (㈜바이오피아)
Publication Information
Korean Journal of Plant Resources / v.17, no.3, 2004 , pp. 239-247 More about this Journal
Abstract
A cyclophilin 1 cDNA clone(GenBank accession no.CF924191) was isolated from the taproot of C. lanceolata and designed as C1CyP1. Determination of the nucleotide sequence of C1CyPl identified an open reading frame of 525bp, which shared high homologies with cyclophilins that were previously reported in other organisms. The C1CyP1 amino acid sequence possesses 7 amino acid residue stretch(KSGKPLH) that is characteristic of plant cytosolic dehydrins. Currently available amino acid residues of plant cyclophilins were compared to examine their phylogenetic relationship to C1CyP1. In the phylogenetic analysis, based on the aligned sequences, C1CyP1 showed high homology with arabidopsis ROC2 and rice CyP1. The transcript that corresponded to C1CyP1 was abundant in callus, but only basal level of transcript was detected in stem, leaf and root. For the study in the defense mechanism against various stresses, we report expression patterns of this gene by quantative RT-PCR.
Keywords
abiotic stress; Codonopsis lanceolata; cyclophilin; RT-PCR;
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