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http://dx.doi.org/10.4490/algae.2016.31.11.31

Proteomic profiles and ultrastructure of regenerating protoplast of Bryopsis plumosa (Chlorophyta)  

Klochkova, Tatyana A. (Department of Biology, Kongju National University)
Kwak, Min Seok (Department of Biology, Kongju National University)
Kim, Gwang Hoon (Department of Biology, Kongju National University)
Publication Information
ALGAE / v.31, no.4, 2016 , pp. 379-390 More about this Journal
Abstract
When a multinucleate cell of Bryopsis plumosa was collapsed by a physical wounding, the extruded protoplasm aggregated into numerous protoplasmic masses in sea water. A polysaccharide envelope which initially covered the protoplasmic mass was peeled off when a cell membrane developed on the surface of protoplast in 12 h after the wounding. Transmission electron microscopy showed that the protoplasmic mass began to form a continuous cell membrane at 6 h after the wounding. The newly generated cell membrane repeated collapse and rebuilding process several times until cell wall developed on the surface. Golgi bodies with numerous vesicles accumulated at the peripheral region of the rebuilding cell at 24 h after the wounding when the cell wall began to develop. Several layers of cell wall with distinctive electron density developed within 48-72 h after the wounding. Proteome profile changed dramatically at each stage of cell rebuilding process. Most proteins, which were up-regulated during the early stage of cell rebuilding disappeared or reduced significantly by 24-48 h. About 70-80% of protein spots detected at 48 h after the wounding were newly appeared ones. The expression pattern of 29 representative proteins was analyzed and the internal amino acid sequences were obtained using mass spectrometry. Our results showed that a massive shift of gene expression occurs during the cell-rebuilding process of B. plumosa.
Keywords
Bryopsis plumosa; cell rebuilding; cell wall; proteome; protoplast; ultrastructure;
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