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http://dx.doi.org/10.4490/algae.2013.28.4.379

Expression of bovine lactoferrin N-lobe by the green alga, Chlorella vulgaris  

Koo, Jungmo (GENOBIO Co.)
Park, Dongjun (GENOBIO Co.)
Kim, Hakeung (GENOBIO Co.)
Publication Information
ALGAE / v.28, no.4, 2013 , pp. 379-387 More about this Journal
Abstract
The purpose of this study was to express bovine lactoferrin N-lobe in Chlorella vulgaris, a green microalga, using the pCAMBIA1304 vector. Chlorella-codon-optimized bovine lactoferrin N-lobe (Lfb-N gene) was cloned in the expression vector pCAMBIA1304, creating the plasmid pCAMLfb-N. pCAMLfb-N was then introduced into C. vulgaris by electro-transformation. Transformants were separated from BG-11 plates containing 20 ${\mu}g\;mL^{-1}$ hygromycin. Polymerase chain reaction was used to screen transformants harboring Lfb-N gene. Finally, total soluble protein was extracted from the transformants, and the expression of Lfb-N protein was detected using western blotting. Using this method, we successfully expressed bovine lactoferrin in C. vulgaris. Therefore, our results suggested that recombinant lactoferrin N-lobe, which has many uses in the biomedical and pharmaceutical industries, can be produced economically.
Keywords
Chlorella vulgaris; electrotransfomation; expression; hygromycin; lactoferrin;
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