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http://dx.doi.org/10.4490/algae.2013.28.4.371

Potential use of ultrasound in antioxidant extraction from Ecklonia cava  

Lee, Seung-Hong (Division of Food Bioscience, Konkuk University)
Kang, Min-Cheol (Department of Marine Life Sciences, Jeju National University)
Moon, Sang-Ho (Korea Nokyong Research Center, Konkuk University)
Jeon, Byong-Tae (Korea Nokyong Research Center, Konkuk University)
Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University)
Publication Information
ALGAE / v.28, no.4, 2013 , pp. 371-378 More about this Journal
Abstract
Water and methanolic extracts of Ecklonia cava, a marine brown alga, were prepared by ultrasonic extraction (UE) and conventional extraction (CE) methods. The radical-scavenging activity and the inhibitory effects against hydrogen peroxide ($H_2O_2$)-induced DNA damage of the extracts were investigated. All extracts prepared by CE exhibited higher total polyphenolic content than that in the extracts prepared by UE. Extraction yield and total phenolic content increased as the UE time increased. The radical-scavenging activities increased as the UE time increased. All extracts prepared by CE exhibited higher 1,1-diphenyl-2-pricrylhydrazyl (DPPH) and hydroxyl radical-scavenging activities than did those prepared by UE. Extracts prepared by UE showed stronger scavenging activities on alkyl radical and $H_2O_2$ than those prepared by CE did. Methanolic extract with UE 12 h (100MEU-12h) and methanolic extract with CE 24 h (100MEC-24h) were selected and evaluated by comet assay for their inhibitory effect against $H_2O_2$-induced DNA damage. 100MEU-12h showed slightly greater protective effect against $H_2O_2$-induced DNA damage than 100MEC-24h. Thus, UE can be effectively used as a seaweed extraction technique, and there is potential for scale-up of the extraction process.
Keywords
Ecklonia cava; extraction yield; polyphenolic content; radical-scavenging activity; ultrasonic extraction;
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1 Shetty, K., Curtis, O. F., Levin, R. E., Witkowsky, R. & Ang, W. 1995. Prevention of vitrification associated with in vitro shoot culture of Oregano. (Origanum vulgare) by Pseudomonas spp. J. Plant Physiol. 3-4:447-451.
2 Soares, J. R., Dinis, T. C. P., Cunha, A. P. & Ameida, L. M. 1997. Antioxidant activities of some extracts of Thymus zygis. Free Radic. Res. 26:469-478.   DOI   ScienceOn
3 Sun, X., Jin, Z., Yang, L., Hao, J., Zu, Y., Wang, W. & Liu, W. 2013. Ultrasonic-assisted extraction of procyanidins using ionic liquid solution from Larix gmelinii Bark. J. Chem. 2013:541037.
4 Valachovic, P., Pechova, A. & Mason, T. J. 2001. Towards the industrial production of medicinal tincture by ultrasound assisted extraction. Ultrason. Sonochem. 8:111-117.   DOI   ScienceOn
5 Vinatoru, M., Toma, M., Radu, O., Filip, P. I., Lazurca, D. & Mason, T. J. 1997. The use of ultrasound for the extraction of bioactive principles from plant materials. Ultrason. Sonochem. 4:135-139.   DOI   ScienceOn
6 Wang, L. & Weller, C. L. 2006. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Technol. 17:300-312.   DOI   ScienceOn
7 Yang, B., Zhao, M., Shi, J., Yang, N. & Jiang, Y. 2007. Effect of ultrasonic treatment on the recovery and DPPH radical scavenging activity of polysaccharides from longan fruit pericarp. Food Chem. 106:685-690.
8 Yang, Y. & Zhang, F. 2008. Ultrasound-assisted extraction of rutin and quercetin from Euonymus alatus (Thunb.) Sieb. Ultrason. Sonochem. 15:308-313.   DOI   ScienceOn
9 Yan, X., Suzuki, M., Ohnishi-Kameyama, M., Sada, Y., Nakanishi, T. & Nagata, T. 1999. Extraction and identification of antioxidants in the roots of yacon (Smallanthus sonchifolius). J. Agric. Food Chem. 47:4711-4713.   DOI   ScienceOn
10 Adebajo, M. O. & Gesser, H. D. 2001. ESR study of alkyl radicals adsorbed on porous Vycor glass. I. Build-up of methyl and ethyl radicals. Appl. Surf. Sci. 171:120-124.   DOI   ScienceOn
11 Ahn, G. -N., Kim, K. -N., Cha, S. -H., Song, C. -B., Lee, J., Heo, M. -S., Yeo, I. -K., Lee, N. -H., Jee, Y. -H., Kim, J. -S., Heu, M. -S. & Jeon, Y. -J. 2007. Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and $H_2O_2$-mediated DNA damage. Eur. Food Res. Technol. 226:71-79.   DOI   ScienceOn
12 Ahn, M. -J., Yoon, K. -D., Min, S. -Y., Lee, J. S., Kim, J. H., Kim, T. G., Kim, S. H., Kim, N. -G., Huh, H. & Kim, J. 2004. Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol. Pharm. Bull. 27:544-547.   DOI   ScienceOn
13 Albu, S., Joyce, E., Paniwnyk, L., Lorimer, J. P. & Mason, T. J. 2004. Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry. Ultrason. Sonochem. 11:261-265.   DOI   ScienceOn
14 Chandler, S. F. & Dodds, J. H. 1983. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum. Plant Cell Rep. 2:205-208.   DOI   ScienceOn
15 Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Mori, H., Nakayama, Y. & Takahashi, M. 1990. Anti-plasmin inhibitor. VI. Structure of phlorofucofuroeckol A, a novel phlorotannin with both dibenzo-1,4-dioxin and dibenzofuran elements, from Ecklonia kurome Okamura. Chem. Pharm. Bull. 38:133-135.   DOI   ScienceOn
16 Heo, S. -J., Park, E. -J., Lee, K. -W. & Jeon, Y. -J. 2005. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresour. Technol. 96:1613-1623.   DOI   ScienceOn
17 Hiramoto, K., Johkoh, H., Sako, K. -I. & Kikugawa, K. 1993. DNA breaking activity of the carbon-centered radical generated from 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH). Free Radic. Res. Commun. 19:323-332.   DOI
18 Ishtiaq, F., Farooq, R., Farooq, U., Farooq, A., Siddique, M., Shah, H., Hassan, M. U. & Shaheen, M. A. 2009. Application of ultrasound in pharmaceutics. World Appl. Sci. J. 6:886-893.
19 Kang, M. C., Ahn, G., Yang, X., Kim, K. N., Kang, S. M., Lee, S. H., Ko, S. C., Ko, J. Y., Kim, D., Kim, Y. T., Jee, Y., Par, S. J. & Jeon, Y. J. 2012. Hepaotprotective effects of dieckol-rich phlorotannins from Ecklonia cava, a brown seaweed, against ethanol induced liver damage in BALB/c mice. Food Chem Toxicol. 50:1986-1991.   DOI   ScienceOn
20 Janzen, E. G., Towner, R. A. & Haire, D. L. 1987. Detection of free radicals generated from the in vitro metabolism of carbon tetrachloride using improved ESR spin trapping techniques. Free Radic. Res. Commun. 3:357-364.   DOI
21 Mason, T. J. & Cordemans, E. D. 1996. Ultrasonic intensification of chemical processing and related operations: a review. Trans. Inst. Chem. Eng. 74:511-516.
22 Mason, T. J., Paniwnyk, L. & Lorimer, J. P. 1996. The uses of ultrasound in food technology. Ultrason. Sonochem. 3:S253-S260.   DOI   ScienceOn
23 Moulton, K. J. & Wang, L. C. 1982. A pilot-plant study of continuous ultrasonic extraction of soybean protein. J. Food Sci. 47:1127-1129.   DOI
24 Müller, H. E. 1985. Detection of hydrogen peroxide produced by microorganism on ABTS-peroxidase medium. Zentralbl. Bakteriol. Mikrobiol. Hyg. Ser. A 259:151-158.
25 Nanjo, F., Goto, K., Seto, R., Suzuki, M., Sakai, M. & Hara, Y. 1996. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radic. Biol. Med. 21:895-902.   DOI   ScienceOn
26 Oki, T., Masuda, M., Furuta, S., Nishiba, Y., Terahara, N. & Suda, I. 2002. Involvement of anthocyanins and other phenolic compounds in radical-scavenging activity of purple-fleshed sweet potato cultivars. J. Food Sci. 67:1752-1756.   DOI   ScienceOn
27 Salisova, M., Toma, S. & Mason, T. J. 1997. Comparison of conventional and ultrasonically assisted extractions of pharmaceutically active compounds from Salvia officinalis. Ultrason. Sonochem. 4:131-134.   DOI   ScienceOn