Browse > Article
http://dx.doi.org/10.4490/algae.2012.27.3.205

Thermostability of a marine polyphenolic antioxidant dieckol, derived from the brown seaweed Ecklonia cava  

Kang, Min-Cheol (Department of Marine Life Sciences, Jeju National University)
Kim, Eun-A (Department of Marine Life Sciences, Jeju National University)
Kang, Sung-Myung (Department of Marine Life Sciences, Jeju National University)
Wijesinghe, W.A.J.P. (Department of Marine Life Sciences, Jeju National University)
Yang, Xiudong (Department of Marine Life Sciences, Jeju National University)
Kang, Na-Lae (Department of Marine Life Sciences, Jeju National University)
Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University)
Publication Information
ALGAE / v.27, no.3, 2012 , pp. 205-213 More about this Journal
Abstract
The thermostability of antioxidant activity of dieckol, a phlorotannin isolated from brown seaweed Ecklonia cava was investigated. The thermostable antioxidant properties of dieckol were evaluated at 30, 60, and $90^{\circ}C$ for 7 days using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, and comparing its performance to that of ascorbic acid. The intracellular reactive oxygen species (ROS) scavenging activity and apoptotic body formation were investigated using DCF-DA assay and nuclear staining with Hoechst 33342, propidium iodide and flow cytometry. Dieckol treated at different temperatures during 7 days showed stable scavenging activities on towards DPPH and hydroxyl radicals. In addition, dieckol showed a stable protective effect against $H_2O_2$-induced apoptotic body formation in Vero cells. On the other hand, the radical scavenging activities and intracellular ROS scavenging activities of ascorbic acid, used as a positive control, were significantly decreased at $60^{\circ}C$ and $90^{\circ}C$ from on the 4th day and 3rd days, respectively. In conclusion, the results indicated that food grade antioxidant extracts containing dieckol derived from E. cava remain a stable during the temperatures encountered during the processing of food and cosmetics.
Keywords
antioxidant; brown seaweed; dieckol; Ecklonia cava; phlorotannin; thermostable;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Choi, Y., Lee, S. M., Chun, J., Lee, H. B. & Lee, J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem. 99:381-387.   DOI   ScienceOn
2 Cordenunsi, B. R., Oliveira do Nascimento, J. R., Genovese, M. I. & Lajolo, F. M. 2002. Influence of cultivar on quality parameters and chemical composition of strawberry fruits grown in Brazil. J. Agric. Food Chem. 50:2581-2586.   DOI   ScienceOn
3 Devi, G. K., Mamivannan, K., Thirumaran, G., Rajathi, F. A. A. R. & Anantharaman, P. 2011. In vitro antioxidant activities of selected seaweeds from Southeast coast of India. Asian Pac. J. Trop. Med. 4:205-211.   DOI   ScienceOn
4 Dykens, J. A., Shick, J. M., Benoit, C., Buettner, G. R. & Winston, G. W. 1992. Oxygen radical production in the sea anemone Anthopleura elegantissima and its endosymbiotic algae. J. Exp. Biol. 168:219-241.
5 Esteve, M. J., Farre, R., Frigola, A. & Clemente, G. 1995. Changes in ascorbic acid content of green asparagus during the harvesting period and storage. J. Agric. Food Chem. 43:2058-2061.   DOI
6 Fritsch, C. W. 1981. Measurements of frying fat deterioration: a brief review. J. Am. Oil Chem. Soc. 58:272-274.   DOI
7 Frei, B., England, L. & Ames, B. N. 1989. Ascorbate is an outstanding antioxidant in human blood plasma. Proc. Natl. Acad. Sci. U. S. A. 86:6377-6381.   DOI   ScienceOn
8 Frolich, I. & Riederer, P. 1995. Free radical mechanisms in dementia of Alzheimer type and the potential for antioxidative treatment. Arzneimittelforschung 45:443-446.
9 Gschwind, M. & Huber, G. 1995. Apoptotic cell death induced by $\beta$-amyloid 1-42 peptide is cell type dependent. J. Neurochem. 65:292-300.
10 Gupta, S., Lakshmi, A. J. & Prakash, J. 2008. Effect of different blanching treatments on ascorbic acid retention in green leafy vegetables. Nat. Prod. Radiance 7:111-116.
11 Ahn, G. -N., Kim, K. -N., Cha, S. -H., Song, C. -B., Lee, J., Heo, M. -S., Yeo, I. -K., Lee, N. -H., Jee, Y. -H., Kim, J. -S., Heu, M. -S. & Jeon, Y. -J. 2007. Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and $H_{2}O_{2}$-mediated DNA damage. Eur. Food Res. Technol. 226:71-79.   DOI
12 Alwazeer, D., Delbeau, C., Divies, C. & Cachon, R. 2003. Use of redox potential modification by gas improves microbial quality, color retention, and ascorbic acid stability of pasteurized orange juice. Int. J. Food Microbiol. 89:21-29.   DOI
13 Amarowicz, R., Naczk, M. & Shahidi, F. 2000. Antioxidant activity of various fractions of non-tannin phenolics of canola hulls. J. Agric. Food Chem. 48:2755-2759.   DOI   ScienceOn
14 Spigno, G., Tramelli, L. & De Faveri, D. M. 2007. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. J. Food Eng. 81:200-208.   DOI   ScienceOn
15 Athukorala, Y. & Jeon, Y. J. 2005. Screening for angiotensin 1-converting enzyme inhibitory activity of Ecklonia cava. J. Food Sci. Nutr. 10:134-139.   DOI
16 Ye, H., Zhou, C., Sun, Y., Zhang, X., Liu, J., Hu, Q. & Zeng, X. 2009. Antioxidant activities in vitro of ethanol extract from brown seaweed Sargassum pallidum. Eur. Food Res. Technol. 230:101-109.   DOI
17 Nicoletti, I., Migliorati, G., Pagliacci, M. C., Grignani, F. G. & Riccardi, C. 1991. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 2:271-279.
18 Park, P. -J., Heo, S. -J., Park, E. -J., Kim, S. -K., Byun, H. -G., Jeon, B. -T. & Jeon, Y. -J. 2005. Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii. J. Agric. Food Chem. 53:6666-6672.   DOI   ScienceOn
19 Park, S. -J., Ahn, G., Lee, N. H., Park, J. W., Jeon, Y. -J. & Jee, Y. 2011. Phloroglucinol (PG) purified from Ecklonia cava attenuates radiation-induced apoptosis in blood lymphocytes and splenocytes. Food Chem. Toxicol. 49:2236- 2242.   DOI
20 Sherwin, E. R. 1990. Antioxidants. In: Branen, R. (Ed.) Food Additives. Marcel Dekker, New York, pp. 139-193.
21 Sukenik, A., Zmora, O. & Carmeli, Y. 1993. Biochemical quality of marine unicellular algae with special emphasis lipid composition.II. Nannochloropsis sp. Aquaculture 117:313-326.   DOI
22 Ukeda, H., Shimamura, T., Tsubouchi, M., Harada, Y., Nakai, Y. & Sawamura, M. 2002. Spectrophotometric assay of superoxide anion formed in Maillard reaction based on highly water-soluble Terazolium salt. Jpn. Soc. Anal. Sci. 18:1151-1154.
23 Wijesinghe, W. A. J. P., Ko, S. -C. & Jeon, Y. -J. 2011. Effect of phlorotannins isolated from Ecklonia cava on angiotensin I-converting enzyme (ACE) inhibitory activity. Nutr. Res. Pract. 5:93-100.   DOI
24 Yang, Y. M., Han, K. Y. & Noh, B. S. 2000. Analysis of lipid oxidation of soybean oil using the portable electronic nose. Food Sci. Biotechnol. 9:146-150.
25 Matsukawa, R., Dubinsky, Z., Kishimoto, E., Masaki, K., Masuda, Y., Takeuchi, T., Chihara, M., Yamamoto, Y., Niki, E. & Karube, I. 1997. A comparison of screening methods for antioxidant activity in seaweeds. J. Appl. Phycol. 9:29-35.   DOI
26 Li, Y., Qian, Z. -J., Ryu, B. M., Lee, S. -H., Kim, M. -M. & Kim, S. -K. 2009. Chemical components and its antioxidant properties in vitro: an edible marine brown alga, Ecklonia cava. Bioorg. Med. Chem. 17:1963-1973.   DOI
27 Lizard, G., Fournel, S., Genestier, L., Dhedin, N., Chaput, C., Flacher, M., Mutin, M., Panaye, G. & Revillard, J. -P. 1995. Kinetics of plasma membrane and mitochondrial alterations in cells undergoing apoptosis. Cytometry 21:275-283.   DOI   ScienceOn
28 Lordan, S., Ross, R. P. & Stanton, C. 2011. Marine bioactives as functional food ingredients: potential to reduce the incidence of chronic diseases. Mar. Drugs 9:1056-1100.   DOI   ScienceOn
29 Mayer, A. M. S. & Hamann, M. T. 2004. Marine pharmacology in 2000: marine compounds with antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiplatelet, antituberculosis, and antiviral activities, affecting the cardiovascular, immune, and nervous systems and other miscellaneous mechanisms of action. Mar. Biotechnol. 6:37-52.   DOI   ScienceOn
30 Nagayama, K., Iwamura, Y., Shibata, T., Hirayama, I. & Nakamura, T. 2002. Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J. Antimicrob. Chemother. 50:889-893.   DOI   ScienceOn
31 Nagy, S. & Smoot, J. M. 1977. Temperature and storage effects on percent retention and percent U. S. recommended dietary allowance of vitamin C in canned single-strength orange juice. J. Agric. Food Chem. 25:135-138.   DOI
32 Nanjo, F., Goto, K., Seto, R., Suzuki, M., Sakai, M. & Hara, Y. 1996. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radical Biol. Med. 6:895-902.
33 Kang, K. A., Lee, K. H., Chae, S., Zhang, R., Jung, M. S., Lee, Y., Kim, H. S., Joo, H. G., Park, J. W., Ham, Y. M., Lee, N. H. & Hyun, J. W. 2005. Eckol isolated from Ecklonia cava attenuates oxidative stress induced cell damage in lung fibroblast cells. FEBS Lett. 579:6295-6304.   DOI   ScienceOn
34 Heo, S. -J & Jeon, Y. -J. 2008. Radical scavenging capacity and cytoprotective effect of enzymatic digests of Ishige okamurae. J. Appl. Phycol. 20:1087-1095.   DOI
35 Heo, S. -J., Yoon, W. -J., Kim, K. -N., Ahn, G. -N., Kang, S. -M., Kang, D. -H., affan, A., Oh, C., Jung, W. -K. & Jeon, Y. -J. 2010. Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharidestimulated RAW 264.7 macrophages. Food Chem. Toxicol. 48:2045-2051.   DOI
36 Im, M. H., Park, Y. -S., Leontowicz, H., Leontowicz, M., Namiesnik, J., Ham, K. -S., Kang, S. -G., Najman, K. & Gorinstein, S. 2011. The thermostability, bioactive compounds and antioxidant activity of some vegetables subjected to different durations of boiling: investigation in vitro. LWT-Food Sci. Technol. 44:92-99.   DOI
37 Kotake-Nara, E., Asai, A. & Nagao, A. 2005. Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells. Cancer Lett. 220:75-84.   DOI   ScienceOn
38 Laguerre, M., Lecomte, J. & Villeneuve, P. 2007. Evaluation of the ability of antioxidants to counteract lipid oxidation: existing methods, new trends and challenges. Prog. Lipid Res. 46:244-282.   DOI   ScienceOn
39 Lee, S. -H., Han, J. -S., Heo, S. -J., Hwang, J. -Y. & Jeon, Y. -J. 2010. Protective effects of dieckol isolated from Ecklonia cava against high glucose-induced oxidative stress in human umbilical vein endothelial cells. Toxicol. In Vitro 24:375-381.   DOI
40 Lehtinen, P., Kiiliäinen, K., Lehtomäki, I. & Laakso, S. 2003. Effect of heat treatment on lipid stability in processed oats. J. Cereal Sci. 37:215-221.   DOI