Browse > Article
http://dx.doi.org/10.4490/ALGAE.2007.22.4.333

Immunomodulating Activity of a Fucoidan Isolated from Korean Undaria pinnatifida Sporophyll  

Yoo, Yung-Choon (Dept. of Microbiology, Konyang University, Konyang University Hospital)
Kim, Woo-Jung (Dept. of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
Kim, So-Yeon (Dept. of Microbiology, Konyang University, Konyang University Hospital)
Kim, Sung-Min (Dept. of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
Chung, Mi-Kyung (Biomedicine Research Group, Hanrib Life Tech, Bio 21 Center)
Park, Joo-Woong (Biomedicine Research Group, Hanrib Life Tech, Bio 21 Center)
Suh, Hyun-Hyo (Dept. of Environmental Engineering, Jinju National University)
Lee, Kyung-Bok (Dept. of Microbiology, Konyang University, Konyang University Hospital)
Park, Yong-Il (Dept. of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
Publication Information
ALGAE / v.22, no.4, 2007 , pp. 333-338 More about this Journal
Abstract
A fucoidan, isolated from Korean Undaria pinnatifida spoprophyll (UP-F), was investigated for its immunomodulating activity on murine macrophages and splenocytes, and its activity was compared with that of fucoidan from Fucus vesiculosus (FV-F). Treatment of UP-F resulted in inhibition of the growth of murine macrophage RAW 264.7 cells, but its cytotoxicity was not observed in normal murine splenocytes. FV-F was shown to be highly cytotoxic to both immune cells, and its cytotoxic activity was higher than that of UP-F. Treatment of UP-F induced TNF-α in a dose-dependent manner from two types of macrophages, RAW 264.7 cells and murine peritoneal macrophages. The TNF-α-inducing activity of UP-F was higher than that of FV-F. UP-F also actively induced chemokines (RANTES and MIP-1α) from RAW 264.7 cells. Furthermore, treatment of UP-F gave rise to activation of murine splenocytes to produce cytokine (IL-6) and chemokines (RANTES and MIP-1α), showing significantly higher activity than that of FV-F. These results indicate that UP-F is less cytotoxic to immune cells than FV-F, and possesses immunomodulating activity to produce cytokines and chemokines from macrophages and splenocytes.
Keywords
fucoidan; immunomodulating activity; Undaria pinnatifida sporophyll;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ostergaard C, Yieng-Kow R.V., Benfield T., Frimodt-Moller N., Espersen F. and Lundgren J.D. 2000. Inhibition of leukocyte entry into the brain by the selectin blocker fucoidin decreases interleukin-1 (IL-1) levels but increases IL-8 levels in cerebrospinal fluid during experimental Pneumococcal meningitis in rabbits. Infect. Immun. 68: 3153-3157   DOI
2 Berteau O. and Mulloy B. 2003. Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharides. Glycobiology 13: 29R-40R   DOI   ScienceOn
3 Choi E. M., Kim A., Kim Y.O . and Hwang J.K. 2005. Immunostimulating activity of arabinogalactan and fucoidan in vitro . J. Med. Food 8: 446-453   DOI   ScienceOn
4 Le Page C., Genin P., Baines M.G. and Hiscoyy J. 2000. Interferon activation and innate immunity. Rev. Immunogenet. 2: 374-386
5 Oomizu S., Yanase Y., Suzuki H., Kameyoshi Y. and Hide M. 2006. Fucoidan prevents C$ \varepsilon$ gemline transcription andNFkB p52 translocation for IgE production in B cells. Biochem. Biophys. Res. Commun. 350: 501-507   DOI   ScienceOn
6 Maruyama H., Tamauchi H., Hashimoto M. and Nakano T. 2003. Antitumor activity and immune response of Mekabu fucoidan extracted from sporophyll of Undaria pinnatifida. In Vivo 17: 245-249
7 McCandless E.L. and Craigie J.S. 1979. Sulfated polysaccharides in red and brown algae . Annu. Rev. Plant Physiol. 30: 41-67   DOI
8 Nishino 1. and Nagumo H. 1991. Structural characterization of a new anticoagulant fucan sulfate from the brown seaweed Ecklonia kurome. Carbohyd. Res. 30: 535-539
9 Beress A., Wassermann O ., Bruhn T. and Beress L. 1993. A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga Fucus vesiculosus. J. Nat. Prod. 56: 478-488   DOI   ScienceOn
10 Daniel R., BerteauO ., Jozefonvicz J. and Goasdoue N. 1999. Degradation of algal (Ascophyllum nodosum) fucoidan by an enzymatic activity contained in digestive glands of the marine mollusc Pecten maxtmus. Carbohyd. Res. 322:291-297   DOI   ScienceOn
11 Fan H., Williams D.L., Zingarelli B., Breuel KF., Teti G., Tempel G.E., Spicher K, Boulay G., Birnbaumer L., Halus hka P.V. and Cook J.A. 2006. Differential regulation of lipopolysaccharide and Gram-positive bacteria induced cytokine and chemokine production in splenocytes by Galphai proteins. Biochim. Biophys. Acta. 1763:1051-1058   DOI   ScienceOn
12 Hoshino T., Hayashi T., Hayashi J., Lee J.B. and Sankawa U. 1998. An antivirally active sulfated polysaccharide from Sargassum horneri (TURNER) C. AGARDH. BioI. Pharm. Bull. 21: 730-734   DOI   ScienceOn
13 Kim W. J., Kim S.M., Kim H.G., Oh H.R., Lee KB., LeeYK and Park Y.I. 2007. Purification and anticoagulant activity of a fucoidan from Korean Undaria pinnatifida Sporophyll. Algae 22:247-252   DOI   ScienceOn
14 Goerdt S., Politz O ., Schledzewski K, Birk R., Gratchev A., Guillot P., Hakiy N., Klemke C.D., Dippel E., Kodelja V. and Orfanos C.E. 1999. Alternative versus classical activation of macrophages. Pathobiology 67:222-226   DOI   ScienceOn