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http://dx.doi.org/10.4490/ALGAE.2007.22.3.247

Purification and Anticoagulant Activity of a Fucoidan from Korean Undaria pinnatifida Sporophyll  

Kim , Woo-Jung (Department of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
Kim, Sung-Min (Department of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
Kim, Hyun-Guell (Department of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
Oh, Hye-Rim (Department of Biochemistry, Konyang university, Konyang University Hospital)
Lee, Kyung-Bok (Department of Biochemistry, Konyang university, Konyang University Hospital)
Lee, Yoo-Kyung (Polar BioCenter, Korea Polar Research Institute, KORDI)
Park, Yong-Il (Department of Biotechnology and The Biomaterial Engineering Research Center, The Catholic University of Korea)
Publication Information
ALGAE / v.22, no.3, 2007 , pp. 247-252 More about this Journal
Abstract
Crude fucoidan was extracted from the sporophyll of Korean Undaria pinnatifida collected at a coastal area ofWando, Korea, mainly by dilute acid extraction, ethanol precipitation, CaCU Precipitation, with an yield of approxi-mately 3.9% in mass. It was further purified by DEAE-cellulose column chromatography and its chemical composi-don and in vitro anticoagulant activity was determined. The average molecular mass of the purified fucoidan wasestimated about 2.1 x 103 kDa by size-fractionation HPLC and it consisted of neutral sugar (52.34% in mass), uronicacid (26.2%), and sulfate esters (7.4%). From the HPAEC-PAD analysis, the monosaccharide composition of thepurified fucoidan was shown to be fucose, galactose, xylose, and mannose, with a molar ratio of 1, 0.2, 0.02, 0.15,respectively, demonstrating that major monosacd-iande was fucose (72.3% in mol percentage) and other sugars,xylose (1.5%), galactose (14.6%), and mannose (10.9%) were present as minor component. The results suggested thatthis fucoidan is a sulfated, U-type fucoidan. The activated partial thrombloplastin time (APTT) assay of the purifiedfucoidan showed that the purified fucoidan elicited anticoagulant activity in a dose-dependent manner. Five jUg ofsporophyll fucoidan delayed the blood clotting time up to 5 times than untreated control and also up to 1.5 timesthan the same amount of the commercial fucoidan, respectively. Although it is preliminary, these results suggestthat the fucoidan of Korean Undaria vinnatifida sporophyll would be promising candidates for the development ofan anticoaeulant.
Keywords
anticoagulant; fucoidan; sporophyll; Undaria pinnatifida;
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1 Zhuang C.. Itoh H., Mizuno T. and Ito H. 1995. Antitumor active fucoidan from the brown seaweed, Umitoranoo (Sargassum thunbergii). Biosci. Biotechnol. Biochem. 59: 563-567   DOI   ScienceOn
2 Anderson L.O., Barrowcliffe T.W., Holmer E., Johnson E.A and Sims G.E.G. 1976. Anticoagulant properties of heparin fractionated by affinity chromatography on matrix-bound antitrombin III and gelfiltrations. Thromb. Res. 9: 575-580   DOI   ScienceOn
3 Beress A, Wassermann O., Bruhn T. and Beress L. 1993. A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga Fucus vesiculosus. J. Nat. Prod. 56: 478-488   DOI   ScienceOn
4 Lee Y.K, Lim D.J., Lee Y.H. and Park Y.I. 2006. Variation in fucoidan contents and monosaccharide compositions of Korean Undaria pinnatifida (Harvey) Suringar (Phaeophyta). Algae 21: 157-160   DOI   ScienceOn
5 Loui J.S., Robert E.H., Leigh S. and Juanita M.S. 1982. Analysis of sulfate in complex carbohydrates. Anal. BioChem. 123: 303-309   DOI   ScienceOn
6 McCandless E.L. and Craigie J.S. 1979. Sulfated polysaccharides in red and brown algae. Annu. Rev. Plant Physiol. 30: 41-67   DOI
7 Millet J., Jouault S.C.. Mauray S., Theveniaux J., Sternberg C. Boisson V.C. and Fischer A.M. 1999. Antithrombotic and anticoagulant activities of a low molecular weight fucoidan by the subcutaneous route. Thromb. Haemost. 81: 391-395   DOI
8 Mori H., Kamei H., Nishide E. and Nisizawa K 1982. Sugar constituents of some sulfated polysaccharides from the sporophylls of wakame (Undaria pinnatifida) and their biological activities. In: Marine algae in pharmaceutical science. Walter de Cruyter, Berlin and New York. pp. 109-121
9 Mourao P.A.S. and Pereira M.S. 1999. Searching for alternatives to heparin: sulfated fucans from marine invertebrates. Trends Cardiovasc. Med. 9: 225-232   DOI   ScienceOn
10 Hoshino T., Hayashi T., Hayashi L Lee J.B. and Sankawa U. 1998. An antivirally active sulfated polysaccharide from Sargassum horneri (TURNER) C. AGARDH. BioI. Pharm. Bull. 21: 730-734   DOI   ScienceOn
11 Nishino T., Fukuda A., Nagumo T. Fujihara M. and Kaji E. 1999. Inhibition of the generation of thrombin and factor Xa by a fucoidan from the brown seaweed Ecklonia kurome. Thromb. Res. 96: 37-49   DOI   ScienceOn
12 Pereira M.S., Mulloy B. and Mour?o P.A. 1999. Structure and anticoagulant activity of sulfated fucans. Comparison between the regular, repetitive, and linear fucans from echinoderms with the more heterogeneous and branched polymers from brown algae. J. Bioi. Chern. 274: 7656-7667   DOI   ScienceOn
13 Nishino T. and Nagumo H. 1991. Structural characterization of a new anticoagulant fucan sulfate from the brown seaweed Ecklonia kurome. Carbohyd. Res. 30: 535-539
14 Riou D., Colliec-Jouault S., Pinczon du sel D., Bosch S., Siavoshian S., LeBert V., Tomasoni C., Sinquin C, Durand P. and Roussakis C. 1996. Antitumor and antiproliferative effects of a fucan extracted from Ascophyllum nodosum against a non-small-cell bronchopulmonary carcinoma line. Anticancer Res. 16: 1213-1218
15 Sakai T., Kimura H., Kojima K., Shimanaka, K. Ikai K. and Kato I. 2003a. Marine bacterial sulfated fucoglucuronomannan (SFGM) lyase digests brown algal SFGM into trisaccharides. Mar. Biotechnol. 5: 70-78   DOI
16 Sakai, T., Ishizuka K. and Kato I. 2003. Isolation and characterization of a fucoidan-degrading marine bacterium. Mar. Biotechnol. 5: 409-416   DOI
17 Berteau a. and Mulloy B. 2003. Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharides. Glycobiology 13: 29R-40R   DOI   ScienceOn
18 Boisson-Vid al C.; Chaubet F., Chevolot L., Sinquin C.; Theveniaux J., Millet J., Sternberg C., Mulloy B. and Fischer A.M. 2000. Relationship between antithrombotic activities of fucans and their structure. Drug Devel. Res. 51: 216-224   DOI   ScienceOn
19 Kim D.S., Lim D.J., Moon S.H., Suh H.H. and Park Y.I. 2004. Purification of fucoidan from Korean sea tangle (Laminaria religosa) and isolation of fucoidan degrading microorganisms. Kor. J. Microbiol. Biotechnol. 32: 362-365
20 Koo J.G., Jo K.S., Do J.R and WOO S.J. 1995. Ioslation and purification of fucoidans from Laminaria religiosa and Undaria pinnatifida in Korea. J. Korean Fish. Soc. 28: 227-236
21 Colliec S., Boisson-Vidal C. and Jozefonvicz J. 1994. A low molecular weight fucoidan fraction from the brown seaweed Pelvetia caniculata. Phytochem. 35: 697-700   DOI   ScienceOn
22 Hahnenberger Rand [akobson A.M. 1991. Antiangiogenic effect of sulphated glycosaminoglycans and polysaccharides in the chick embryo chorioallantoic membrane. Glycoconjugate J. 8: 350-353   DOI
23 Daniel R., Berteau a., Jozefonvicz J. and Goasdoue N. 1999. Degradation of algal (Ascophyllum nodosum) fucoidan by an enzymatic activity contained in digestive glands of the marine mollusc Pecten maximus. Carbohyd. Res. 322: 291-297   DOI   ScienceOn
24 Dubois M., Gilles K. A, Hamilton J. K, Rebers P.A. and Smith F. 1956. A colorimeteric method for determination of sugars and related substances. Analysis Chem. 28: 350-356   DOI
25 Fisher K.G. 2007. Essentials of anticoagulation in hemodialysis. Hemodialysis International 11: 178-189   DOI   ScienceOn
26 McClure M.O., Moore J.P., Blanc D.F., Scotting P., Cook G.M., Keynes R.J., Weber J.N., Davies D. and Weiss R.A 1992. Investigation into the mechanism by which sulfated polysaccharides inhibit HIV- infection in vitro. AIDS Res. Hum. Retrov. 8: 19-26   DOI   ScienceOn
27 Ostergaard c., Yieng-Kow R.V., Benfield T., Frimodt-Moller N., Espersen F. and Lundgren J.D. 2000. Inhibition of leukocyte entry into the brain by the selectin blocker fucoidin decreases interleukin-1 (IL-1) levels but increases IL-8 levels in cerebrospinal fluid during experimental Pneumococcal meningitis in rabbits. Infect. Immun. 68: 3153-3157   DOI
28 Patankar S., Oehniger S., Barnett T., Williams R. L. and Clark G.F. 1993. A revised structure for fucoidan may explain some of its biological activities. J. Bioi. Chem. 268: 21770-21776
29 Chevolot L., Foucault A, Chaubet F., Kervarec N., Sinquin C; Fisher A.M. and Boisson-Vidal C. 1999. Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity. Carbohyd. Res. 319: 154-165   DOI   ScienceOn
30 Bradford M.M. 1976. A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254   DOI   ScienceOn
31 Bitter T. and Muir H.M. 1962. A modified uronic acid carbazole reaction, Anal. Biochem. 4: 330-334   DOI   ScienceOn
32 Nishino T., Nishioka C, Ura H. and Nagumo T. 1994. Isolation and partial characterization of a novel amino sugar-containing fucan sulfate from commercial Fucus vesiculosus fucoidan. Carbohydr. Res. 255: 213-224   DOI   ScienceOn